Anti-DHFR Antibody (RACO0356)
- SKU:
- RACO0356
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Description
| 商品名: | DHFR Antibody |
| Product SKU: | RACO0356 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IHC, IP |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000 |
| 反応性: | Human, Mouse, Rat |
| 免疫原: | A synthesized peptide derived from human DHFR |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2. |
| シノニム: | Dihydrofolate reductase (EC 1.5.1.3), DHFR |
 | IHC image of RACO0356 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | IHC image of RACO0356 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | Western Blot. Positive WB detected in Hela whole cell lysate Jurkat whole cell lysate K562 whole cell lysate Raji whole cell lysate 293T whole cell lysate Rat heart tissue, Mouse kidney tissue, Mouse liver tissue. All lanes DHFR antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 22, 16 kDa. Observed band size: 22 kDa. |
| UniProt Protein Function: | DHFR: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1. Defects in DHFR are the cause of megaloblastic anemia due to dihydrofolate reductase deficiency (DHFRD). DHFRD is an inborn error of metabolism, characterized by megaloblastic anemia and/or pancytopenia, severe cerebral folate deficiency, and cerebral tetrahydrobiopterin deficiency. Clinical features include variable neurologic symptoms, ranging from severe developmental delay and generalized seizures in infancy, to childhood absence epilepsy with learning difficulties, to lack of symptoms. Belongs to the dihydrofolate reductase family. |
| UniProt Protein Details: | Protein type:Cofactor and Vitamin Metabolism - one carbon pool by folate; Oxidoreductase; EC 1.5.1.3; Cofactor and Vitamin Metabolism - folate biosynthesis Chromosomal Location of Human Ortholog: 5q14.1 Cellular Component: nucleoplasm; cytosol Molecular Function:mRNA binding; drug binding; dihydrofolate reductase activity; NADP binding Biological Process: tetrahydrofolate metabolic process; vitamin metabolic process; one-carbon compound metabolic process; nitric oxide metabolic process; G1/S-specific transcription in mitotic cell cycle; tetrahydrofolate biosynthetic process; nucleotide biosynthetic process; response to methotrexate; regulation of nitric-oxide synthase activity; folic acid metabolic process; mitotic cell cycle; glycine biosynthetic process; water-soluble vitamin metabolic process; G1/S transition of mitotic cell cycle Disease: Megaloblastic Anemia Due To Dihydrofolate Reductase Deficiency |
| UniProt Code: | P00374 |
| NCBI GenInfo Identifier: | 118992 |
| NCBI Gene ID: | |
| NCBI Accession: | P00374.2 |
| Molecular Weight: | |
| NCBI Full Name: | Dihydrofolate reductase |
| UniProt Protein Name: | Dihydrofolate reductase |
| Protein Family: | Viral dihydrofolate reductase |
| UniProt Gene Name: | DHFR |
| UniProt Entry Name: | DYR_HUMAN |