Anti-DGUOK Antibody (CAB6898)
- SKU:
- CAB6898
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-DGUOK Antibody |
| 抗体コード: | CAB6898 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-277 of human DGUOK (NP_550438.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Jurkat, HepG2, Mouse heart, Mouse kidney, Rat liver, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-277 of human DGUOK (NP_550438.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | RLSI EGNI AVGK STFV KLLT KTYP EWHV ATEP VATW QNIQ AAGT QKAC TAQS LGNL LDMM YREP ARWS YTFQ TFSF LSRL KVQL EPFP EKLL QARK PVQI FERS VYSD RYIF AKNL FENG SLSD IEWH IYQD WHSF LLWE FASR ITLH GFIY LQAS PQVC LKRL YQRA REEE KGIE LAYL EQLH GQHE AWLI HKTT KLHF EALM NIPV LVLD VNDD FSEE VTKQ EDLM REVN TFVK NL |
| 遺伝子ID: | 1716 |
| Uniprot: | Q16854 |
| セルラーロケーション: | Mitochondrion |
| 計算された分子量: | 9kDa/17kDa/21kDa/27kDa/29kDa/32kDa |
| 観察された分子量: | 40kDa |
| 同義語: | DGUOK, MTDPS3, NCPH, PEOB4, dGK |
| バックグラウンド: | In mammalian cells, the phosphorylation of purine deoxyribonucleosides is mediated predominantly by two deoxyribonucleoside kinases, cytosolic deoxycytidine kinase and mitochondrial deoxyguanosine kinase. The protein encoded by this gene is responsible for phosphorylation of purine deoxyribonucleosides in the mitochondrial matrix. In addition, this protein phosphorylates several purine deoxyribonucleoside analogs used in the treatment of lymphoproliferative disorders, and this phosphorylation is critical for the effectiveness of the analogs. Alternative splice variants encoding different protein isoforms have been described for this gene. |
| UniProt Protein Function: | DGUOK: Required for the phosphorylation of several deoxyribonucleosides and certain nucleoside analogs widely employed as antiviral and chemotherapeutic agents. Defects in DGUOK are the cause of mitochondrial DNA depletion syndrome type 3 (MTDPS3). A disorder characterized by onset in infancy of progressive liver failure, hypoglycemia, increased lactate in body fluids, and neurologic abnormalities including hypotonia, encephalopathy, peripheral neuropathy. Affected tissues show both decreased activity of the mtDNA-encoded respiratory chain complexes and mtDNA depletion. Belongs to the DCK/DGK family. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Nucleotide Metabolism - purine; EC 2.7.1.113; Mitochondrial; Kinase, other Chromosomal Location of Human Ortholog: 2p13 Cellular Component: mitochondrion; mitochondrial matrix; nucleus; cytosol Molecular Function:deoxyguanosine kinase activity; nucleoside kinase activity; ATP binding Biological Process: purine deoxyribonucleoside metabolic process; nucleotide biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; dGTP metabolic process; guanosine metabolic process; protein amino acid phosphorylation; purine salvage; purine base metabolic process; deoxyribonucleoside monophosphate biosynthetic process Disease: Mitochondrial Dna Depletion Syndrome 3 (hepatocerebral Type) |
| NCBI Summary: | In mammalian cells, the phosphorylation of purine deoxyribonucleosides is mediated predominantly by two deoxyribonucleoside kinases, cytosolic deoxycytidine kinase and mitochondrial deoxyguanosine kinase. The protein encoded by this gene is responsible for phosphorylation of purine deoxyribonucleosides in the mitochondrial matrix. In addition, this protein phosphorylates several purine deoxyribonucleoside analogs used in the treatment of lymphoproliferative disorders, and this phosphorylation is critical for the effectiveness of the analogs. Alternative splice variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q16854 |
| NCBI GenInfo Identifier: | 23503050 |
| NCBI Gene ID: | 1716 |
| NCBI Accession: | Q16854.2 |
| UniProt Secondary Accession: | Q16854,P78532, Q16759, Q4ZG09, Q7L1W9, Q96BC1, |
| UniProt Related Accession: | Q16854 |
| Molecular Weight: | 277 |
| NCBI Full Name: | Deoxyguanosine kinase, mitochondrial |
| NCBI Synonym Full Names: | deoxyguanosine kinase |
| NCBI Official Symbol: | DGUOK |
| NCBI Official Synonym Symbols: | dGK; MTDPS3 |
| NCBI Protein Information: | deoxyguanosine kinase, mitochondrial |
| UniProt Protein Name: | Deoxyguanosine kinase, mitochondrial |
| UniProt Gene Name: | DGUOK |
| UniProt Entry Name: | DGUOK_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DGUOK antibody (CAB6898) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded mouse brain using DGUOK antibody (CAB6898) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse stomach using DGUOK antibody (CAB6898) at dilution of 1:100 (40x lens). |
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