Anti-DGCR8 Antibody (RACO0145)
- SKU:
- RACO0145
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | DGCR8 Antibody |
| Product SKU: | RACO0145 |
| サイズ: | 50ul |
| 宿主種: | Human |
| 申し込み: | ELISA, WB |
| 推奨される希釈: | WB:1:500-1:5000 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human DGCR8 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal (By similarity). |
| シノニム: | Microprocessor complex subunit DGCR8, DiGeorge syndrome critical region 8, DGCR8, C22orf12, DGCRK6, LP4941 |
 | Western Blot. Positive WB detected in K562 whole cell lysate All lanes DGCR8 antibody at 2.65µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 87, 33, 83 KDa. Observed band size: 100 KDa. |
| UniProt Protein Function: | DGCR8: a double strand RNA binding protein (dsRBP) which is essential for the first processing step of primary miRNAs (pri-miRNAs). Apparently binds and is activated by heme. A nuclear complex of Drosha with DGCR8 cleaves pri-miRNA substrates into 60-70 nucleotide intermediates (pre-miRNA), thus initiating the microRNA maturation process. Drosha provides the catalytic site for this cleavage, whereas DGCR8 (Pasha) anchors substrate pri-miRNAs. Pre-miRNAs are then processed by the cytoplasmic Dicer-TRBP complex to approximately 20-22 nt mature miRNAs. |
| UniProt Protein Details: | Protein type:RNA processing; RNA-binding; Nucleolus Chromosomal Location of Human Ortholog: 22q11.2 Cellular Component: cytoplasm; microtubule cytoskeleton; nucleoplasm; nucleus Molecular Function:double-stranded RNA binding; heme binding; protein binding; protein homodimerization activity; ribonuclease III activity Biological Process: primary microRNA processing; RNA-mediated gene silencing Disease: Velocardiofacial Syndrome |
| NCBI Summary: | This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010] |
| UniProt Code: | Q8WYQ5 |
| NCBI GenInfo Identifier: | 23813990 |
| NCBI Gene ID: | 54487 |
| NCBI Accession: | Q8WYQ5.1 |
| UniProt Secondary Accession: | Q8WYQ5,Q6DCB2, Q6MZE9, Q6Y2L0, Q96G39, Q96GP8, Q9H6L8 Q9H6T7, Q9NRW2, B2R8G1, |
| UniProt Related Accession: | Q8WYQ5 |
| Molecular Weight: | 82,750 Da |
| NCBI Full Name: | Microprocessor complex subunit DGCR8 |
| NCBI Synonym Full Names: | DGCR8 microprocessor complex subunit |
| NCBI Official Symbol: | DGCR8 |
| NCBI Official Synonym Symbols: | Gy1; pasha; DGCRK6; C22orf12 |
| NCBI Protein Information: | microprocessor complex subunit DGCR8 |
| UniProt Protein Name: | Microprocessor complex subunit DGCR8 |
| UniProt Synonym Protein Names: | DiGeorge syndrome critical region 8 |
| Protein Family: | Microprocessor complex |
| UniProt Gene Name: | DGCR8 |
| UniProt Entry Name: | DGCR8_HUMAN |