Anti-DDX5 Antibody (RACO0381)
- SKU:
- RACO0381
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Application:
- FC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | DDX5 Antibody |
| Product SKU: | RACO0381 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IHC, IF, FC |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, FC:1:20-1:200 |
| 反応性: | Human, Mouse |
| 免疫原: | A synthesized peptide derived from human DDX5 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for estrogen receptor ESR1 and androgen receptor AR. Increases ESR1 AF-1 domain-mediated transactivation and ESR1 AF-1 and AF-2 domains transcriptional synergistic activity. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms. |
| シノニム: | Probable ATP-dependent RNA helicase DDX5 (EC 3.6.4.13) (DEAD box protein 5) (RNA helicase p68), DDX5, G17P1 HELR HLR1 |
 | IHC image of RACO0381 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | Immunofluorescence staining of HepG2 Cells with RACO0381 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
 | Western Blot. Positive WB detected in HepG2 whole cell lysate HT-29 whole cell lysate 293T whole cell lysate Jurkat whole cell lysate Mouse brain tissue. All lanes DDX5 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 70, 61 kDa. Observed band size: 70 kDa. |
| UniProt Protein Function: | DDX5: a putative ATP-dependent RNA helicase. A proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Belongs to the DEAD box helicase family. DDX5/DDX17 subfamily. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. |
| UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; Spliceosome; RNA-binding; RNA splicing; EC 3.6.4.13; Nucleolus; Helicase Chromosomal Location of Human Ortholog: 17q21 Cellular Component: nucleoplasm; membrane; nucleolus; ribonucleoprotein complex; nucleus Molecular Function:calmodulin binding; protein binding; enzyme binding; androgen receptor binding; transcription coactivator activity; estrogen receptor binding; calcium-dependent protein binding; ATP-dependent RNA helicase activity; RNA helicase activity; ATP binding Biological Process: nuclear mRNA splicing, via spliceosome; circadian rhythm; regulation of osteoblast differentiation; transcription, DNA-dependent; in utero embryonic development; positive regulation of estrogen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; positive regulation of DNA damage response, signal transduction by p53 class mediator; cell growth; regulation of viral genome replication; regulation of alternative nuclear mRNA splicing, via spliceosome |
| NCBI Summary: | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P17844 |
| NCBI GenInfo Identifier: | 129383 |
| NCBI Gene ID: | 1655 |
| NCBI Accession: | P17844.1 |
| UniProt Secondary Accession: | P17844,O75681, Q53Y61, B4DLW8, B5BU21, D3DU32, E7ETL9 |
| UniProt Related Accession: | P17844 |
| Molecular Weight: | 60,563 Da |
| NCBI Full Name: | Probable ATP-dependent RNA helicase DDX5 |
| NCBI Synonym Full Names: | DEAD (Asp-Glu-Ala-Asp) box helicase 5 |
| NCBI Official Symbol: | DDX5 |
| NCBI Official Synonym Symbols: | p68; HLR1; G17P1; HUMP68 |
| NCBI Protein Information: | probable ATP-dependent RNA helicase DDX5; DEAD box-5; RNA helicase p68; DEAD box protein 5; DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD) |
| UniProt Protein Name: | Probable ATP-dependent RNA helicase DDX5 |
| UniProt Synonym Protein Names: | DEAD box protein 5; RNA helicase p68 |
| Protein Family: | Probable ATP-dependent RNA helicase |
| UniProt Gene Name: | DDX5 |
| UniProt Entry Name: | DDX5_HUMAN |