Anti-DCTN2 Antibody (CAB2200)
- SKU:
- CAB2200
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-DCTN2 Antibody |
| 抗体コード: | CAB2200 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 50-320 of human DCTN2 (NP_006391.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | MCF7 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 50-320 of human DCTN2 (NP_006391.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | HIIV NPNA AYDK FKDK RVGT KGLD FSDR IGKT KRTG YESG EYEM LGEG LGVK ETPQ QKYQ RLLH EVQE LTTE VEKI KTTV KESA TEEK LTPV LLAK QLAA LKQQ LVAS HLEK LLGP DAAI NLTD PDGA LAKR LLLQ LEAT KNSK GGSG GKTT GTPP DSSL VTYE LHSR PEQD KFSQ AAKV AELE KRLT ELET AVRC DQDA QNPL SAGL QGAC LMET VELL QAKV SALD LAVL DQVE ARLQ SVLG KVNE IAKH KASV EDAD TQSK VHQL YET |
| 遺伝子ID: | 10540 |
| Uniprot: | Q13561 |
| セルラーロケーション: | Cytoplasm, Membrane, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center |
| 計算された分子量: | 44kDa |
| 観察された分子量: | 44kDa |
| 同義語: | DCTN2, DCTN50, DYNAMITIN, HEL-S-77, RBP50 |
| バックグラウンド: | This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| UniProt Protein Function: | dynactin 2: Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development. Belongs to the dynactin subunit 2 family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cell cycle regulation; Motor; Microtubule-binding; Cytoskeletal Chromosomal Location of Human Ortholog: 12q13.3 Cellular Component: kinetochore; dynein complex; centrosome; microtubule; dynactin complex; growth cone; membrane; cytoplasm; cytosol; vesicle Molecular Function:protein binding; spectrin binding; motor activity Biological Process: cell proliferation; mitosis; mitotic spindle organization and biogenesis; metabolic process; organelle organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class II; mitotic cell cycle; G2/M transition of mitotic cell cycle; melanosome transport |
| NCBI Summary: | This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2012] |
| UniProt Code: | Q13561 |
| NCBI GenInfo Identifier: | 22096346 |
| NCBI Gene ID: | 10540 |
| NCBI Accession: | Q13561.4 |
| UniProt Secondary Accession: | Q13561,Q86YN2, Q9BW17, B2RBK5, |
| UniProt Related Accession: | Q13561 |
| Molecular Weight: | 401 |
| NCBI Full Name: | Dynactin subunit 2 |
| NCBI Synonym Full Names: | dynactin 2 (p50) |
| NCBI Official Symbol: | DCTN2 |
| NCBI Official Synonym Symbols: | RBP50; DCTN50; HEL-S-77; DYNAMITIN |
| NCBI Protein Information: | dynactin subunit 2; p50 dynamitin; dynactin complex 50 kD subunit; dynactin complex 50 kDa subunit; epididymis secretory protein Li 77; 50 kD dynein-associated polypeptide; 50 kDa dynein-associated polypeptide |
| UniProt Protein Name: | Dynactin subunit 2 |
| UniProt Synonym Protein Names: | 50 kDa dynein-associated polypeptide; Dynactin complex 50 kDa subunit; DCTN-50; p50 dynamitin |
| Protein Family: | Dynactin |
| UniProt Gene Name: | DCTN2 |
| UniProt Entry Name: | DCTN2_HUMAN |
 | Western blot analysis of extracts of MCF-7 cells, using DCTN2 antibody (CAB2200) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded human kidney using DCTN2 antibody (CAB2200) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using DCTN2 antibody (CAB2200) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using DCTN2 antibody (CAB2200) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using DCTN2 antibody (CAB2200). Blue: DAPI for nuclear staining. |
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