Anti-CYP17A1 Antibody (RACO0483)
- SKU:
- RACO0483
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | CYP17A1 Antibody |
| Product SKU: | RACO0483 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IHC, IF |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200 |
| 反応性: | Human, Rat |
| 免疫原: | A synthesized peptide derived from human Cytochrome P450 17A1 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. |
| シノニム: | Steroid 17-alpha-hydroxylase/17,20 lyase (EC 1.14.14.19) (17-alpha-hydroxyprogesterone aldolase) (EC 1.14.14.32) (CYPXVII) (Cytochrome P450 17A1) (Cytochrome P450-C17) (Cytochrome P450c17) (Steroid 17-alpha-monooxygenase), CYP17A1, CYP17 S17AH |
 | IHC image of RACO0483 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | Immunofluorescence staining of Hela Cells with RACO0483 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
 | Western Blot. Positive WB detected in HepG2 whole cell lysate Rat Heart whole cell lysate Rat Brain whole cell lysate All lanes CYP17A1 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 58 kDa. Observed band size: 58 kDa. |
| UniProt Code: | P05093 |
| NCBI GenInfo Identifier: | 117283 |
| NCBI Gene ID: | 1586 |
| NCBI Accession: | P05093.1 |
| UniProt Secondary Accession: | P05093,Q5TZV7, |
| UniProt Related Accession: | P05093 |
| Molecular Weight: | 57,371 Da |
| NCBI Full Name: | Steroid 17-alpha-hydroxylase/17,20 lyase |
| NCBI Synonym Full Names: | cytochrome P450 family 17 subfamily A member 1 |
| NCBI Official Symbol: | CYP17A1 |
| NCBI Official Synonym Symbols: | CPT7; CYP17; S17AH; P450C17 |
| NCBI Protein Information: | steroid 17-alpha-hydroxylase/17,20 lyase |
| UniProt Protein Name: | Steroid 17-alpha-hydroxylase/17,20 lyase |
| UniProt Synonym Protein Names: | 17-alpha-hydroxyprogesterone aldolase (EC:1.14.14.321 PublicationManual assertion based on experiment iniRef.10"Structures of cytochrome P450 17A1 with prostate cancer drugs abiraterone and TOK-001."DeVore N.M., Scott E.E.Nature 482:116-119(2012) [PubMed] [Europe PMC] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 24-508 IN COMPLEXES WITH HEME; ABIRATERONE AND TOK-001, FUNCTION, CATALYTIC ACTIVITY, COFACTOR. |
| Protein Family: | Steroid 17-alpha-hydroxylase/17,20 lyase |
| UniProt Gene Name: | CYP17A1 |