Anti-COPS3 Antibody (CAB7017)
- SKU:
- CAB7017
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-COPS3 Antibody |
| 抗体コード: | CAB7017 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 194-423 of human COPS3 (NP_003644.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | SW480, HeLa, 293T, Mouse heart, Rat heart, Rat brian |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 194-423 of human COPS3 (NP_003644.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | GLKN FERA LYFY EQAI TTPA MAVS HIML ESYK KYIL VSLI LLGK VQQL PKYT SQIV GRFI KPLS NAYH ELAQ VYST NNPS ELRN LVNK HSET FTRD NNMG LVKQ CLSS LYKK NIQR LTKT FLTL SLQD MASR VQLS GPQE AEKY VLHM IEDG EIFA SINQ KDGM VSFH DNPE KYNN PAML HNID QEML KCIE LDER LKAM DQEI TVNP QFVQ KSMG SQED DSGN KPSS YS |
| 遺伝子ID: | 8533 |
| Uniprot: | Q9UNS2 |
| セルラーロケーション: | Cytoplasm, Nucleus |
| 計算された分子量: | 45kDa/47kDa |
| 観察された分子量: | 48kDa |
| 同義語: | COPS3, CSN3, SGN3 |
| バックグラウンド: | The protein encoded by this gene possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Several transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | COPS3: Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Belongs to the CSN3 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 17p11.2 Cellular Component: nucleoplasm; signalosome; cytoplasm; nucleus Molecular Function:protein binding Biological Process: ubiquitin-dependent protein catabolic process; response to light stimulus; cullin deneddylation; in utero embryonic development; signal transduction |
| NCBI Summary: | The protein encoded by this gene possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] |
| UniProt Code: | Q9UNS2 |
| NCBI GenInfo Identifier: | 55976621 |
| NCBI Gene ID: | 8533 |
| NCBI Accession: | Q9UNS2.3 |
| UniProt Secondary Accession: | Q9UNS2,O43191, Q7LDR6, B2R683, B4DY81, |
| UniProt Related Accession: | Q9UNS2 |
| Molecular Weight: | 423 |
| NCBI Full Name: | COP9 signalosome complex subunit 3 |
| NCBI Synonym Full Names: | COP9 signalosome subunit 3 |
| NCBI Official Symbol: | COPS3 |
| NCBI Official Synonym Symbols: | CSN3; SGN3 |
| NCBI Protein Information: | COP9 signalosome complex subunit 3; signalosome subunit 3; COP9 complex subunit 3; JAB1-containing signalosome subunit 3; COP9 constitutive photomorphogenic homolog subunit 3 |
| UniProt Protein Name: | COP9 signalosome complex subunit 3 |
| UniProt Synonym Protein Names: | JAB1-containing signalosome subunit 3 |
| UniProt Gene Name: | COPS3 |
| UniProt Entry Name: | CSN3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using COPS3 antibody (CAB7017) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of MCF7 cells using COPS3 antibody (CAB7017). Blue: DAPI for nuclear staining. |
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