Anti-CKMT1A Antibody (CAB5233)
- SKU:
- CAB5233
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-CKMT1A Antibody |
| 抗体コード: | CAB5233 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 258-417 of human CKMT1A (NP_001015001.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse heart, Rat heart, Rat testis |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 258-417 of human CKMT1A (NP_001015001.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | FLIW VNEE DHTR VISM EKGG NMKR VFER FCRG LKEV ERLI QERG WEFM WNER LGYI LTCP SNLG TGLR AGVH IKLP LLSK DSRF PKIL ENLR LQKR GTGG VDTA ATGG VFDI SNLD RLGK SEVE LVQL VIDG VNYL IDCE RRLE RGQD IRIP TPVI HTKH |
| 遺伝子ID: | 548596 |
| Uniprot: | P12532 |
| セルラーロケーション: | Intermembrane side, Mitochondrion inner membrane, Peripheral membrane protein |
| 計算された分子量: | 47kDa/50kDa |
| 観察された分子量: | 47kDa |
| 同義語: | CKMT1A, CKMT1, U-MtCK, mia-CK |
| バックグラウンド: | Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. |
| UniProt Protein Function: | Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. |
| NCBI Summary: | Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P12532 |
| NCBI GenInfo Identifier: | 125315 |
| NCBI Gene ID: | 548596 |
| NCBI Accession: | P12532.1 |
| UniProt Secondary Accession: | P12532,Q0VAM3, Q32NF6, Q53FC4, B4DIT8, B7ZA09, |
| UniProt Related Accession: | P12532 |
| Molecular Weight: | 50,421 Da |
| NCBI Full Name: | Creatine kinase U-type, mitochondrial |
| NCBI Synonym Full Names: | creatine kinase, mitochondrial 1A |
| NCBI Official Symbol: | CKMT1A |
| NCBI Official Synonym Symbols: | CKMT1; U-MtCK; mia-CK |
| NCBI Protein Information: | creatine kinase U-type, mitochondrial |
| UniProt Protein Name: | Creatine kinase U-type, mitochondrial |
| UniProt Synonym Protein Names: | Acidic-type mitochondrial creatine kinase; Mia-CK; Ubiquitous mitochondrial creatine kinase; U-MtCK |
| UniProt Gene Name: | CKMT1A |
| UniProt Entry Name: | KCRU_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CKMT1A Antibody (CAB5233) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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