Anti-CENPH Antibody (CAB8372)
- SKU:
- CAB8372
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-CENPH Antibody |
| 抗体コード: | CAB8372 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-247 of human CENPH (NP_075060.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | HeLa, NIH/3T3, 293T, OVCAR3, A673 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-247 of human CENPH (NP_075060.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MEEQ PQMQ DADE PADS GGEG RAGG PPQV AGAQ AACS EDRM TLLL RLRA QTKQ QLLE YKSM VDAS EEKT PEQI MQEK QIEA KIED LENE IEEV KVAF EIKK LALD RMRL STAL KKNL EKIS RQSS VLMD NMKH LLEL NKLI MKSQ QESW DLEE KLLD IRKK RLQL KQAS ESKL LEIQ TEKN KQKI DLDS MENS ERIK IIRQ NLQM EIKI TTVI QHVF QNLI LGSK VNWA EDPA LKEI VLQL EKNV DMM |
| 遺伝子ID: | 64946 |
| Uniprot: | Q9H3R5 |
| セルラーロケーション: | Chromosome, Nucleus, centromere, kinetochore |
| 計算された分子量: | 28kDa |
| 観察された分子量: | 38kDa |
| 同義語: | CENPH |
| バックグラウンド: | Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. |
| UniProt Protein Function: | CENPH: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate. Belongs to the centromere protein H family. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 5p15.2 Cellular Component: cytosol; kinetochore; nucleoplasm; nucleus Molecular Function:kinetochore binding; protein binding Biological Process: DNA replication-independent nucleosome assembly at centromere; kinetochore organization and biogenesis; sister chromatid cohesion |
| NCBI Summary: | Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9H3R5 |
| NCBI GenInfo Identifier: | 74733576 |
| NCBI Gene ID: | 64946 |
| NCBI Accession: | Q9H3R5.1 |
| UniProt Secondary Accession: | Q9H3R5,A8K3Y1, |
| UniProt Related Accession: | Q9H3R5 |
| Molecular Weight: | 28,481 Da |
| NCBI Full Name: | Centromere protein H |
| NCBI Synonym Full Names: | centromere protein H |
| NCBI Official Symbol: | CENPH |
| NCBI Protein Information: | centromere protein H |
| UniProt Protein Name: | Centromere protein H |
| UniProt Synonym Protein Names: | Interphase centromere complex protein 35 |
| UniProt Gene Name: | CENPH |
| UniProt Entry Name: | CENPH_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CENPH antibody (CAB8372) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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