Anti-CDK4 Antibody (RACO0272)
- SKU:
- RACO0272
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- ELISA
- Conjugation:
- Unconjugated
Description
| 商品名: | CDK4 Antibody |
| Product SKU: | RACO0272 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IP |
| 推奨される希釈: | WB:1:500-1:5000, IP:1:200-1:1000 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human CDK4 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. |
| シノニム: | Cyclin-dependent kinase 4 (EC 2.7.11.22) (Cell division protein kinase 4) (PSK-J3), CDK4 |
 | Immunoprecipitating CDK4 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of RACO0272 in Hela whole cell lysate For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/1500). Lane 2:RACO0272(2µg)+ Hela whole cell lysate (500µg). Lane 3: Hela whole cell lysate (10µg). |
 | Western Blot. Positive WB detected in Hela whole cell lysate HepG2 whole cell lysate Jurkat whole cell lysate 293 whole cell lysate MCF-7 whole cell lysate PC-3 whole cell lysate All lanes CDK4 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 34, 21 kDa. Observed band size: 34 kDa. |
| UniProt Protein Function: | CDK4: a protein kinase of the CDK family that is important for cell cycle G1 phase progression. Its activity is restricted to the G1-S phase. Controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). Phosphorylates the retinoblastoma gene product (Rb). Point mutations found in somatic and familial melanoma. Amplified in sarcomas, glioma and lymphoma. Amplified, methylated or deleted in head and neck squamous cell carcinoma. Overexpression drives epithelial tumors in mice. Disruption makes mice resistant to cancer. Inhibitor: PD332991. |
| UniProt Protein Details: | Protein type:EC 2.7.11.22; Kinase, protein; Protein kinase, CMGC; Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); CMGC group; CDK family; CDK4 subfamily; CDK/CDK4 subfamily Chromosomal Location of Human Ortholog: 12q14 Cellular Component: nucleoplasm; transcription factor complex; nuclear membrane; tight junction; perinuclear region of cytoplasm; nucleolus; cyclin-dependent protein kinase holoenzyme complex; nucleus; chromatin; cytosol Molecular Function:protein binding; cyclin binding; cyclin-dependent protein kinase activity; protein complex binding; cyclin-dependent protein kinase regulator activity; ATP binding Biological Process: circadian rhythm; lens development in camera-type eye; response to drug; establishment and/or maintenance of chromatin architecture; organ regeneration; positive regulation of cell size; positive regulation of translation; positive regulation of apoptosis; response to toxin; response to testosterone stimulus; signal transduction; protein amino acid phosphorylation; positive regulation of fibroblast proliferation; response to hyperoxia; regulation of gene expression; cell division; response to lead ion; positive regulation of cell proliferation; mitotic cell cycle; regulation of protein kinase activity; G1/S transition of mitotic cell cycle Disease: Melanoma, Cutaneous Malignant, Susceptibility To, 3 |
| NCBI Summary: | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P11802 |
| NCBI GenInfo Identifier: | 1168867 |
| NCBI Gene ID: | 1019 |
| NCBI Accession: | P11802.2 |
| UniProt Secondary Accession: | P11802,O00576, Q6FG61, B2R9A0, B4DNF9, |
| UniProt Related Accession: | P11802 |
| Molecular Weight: | Calculated MW: 20kDa/33kDaObserved MW: 34kDa |
| NCBI Full Name: | Cyclin-dependent kinase 4 |
| NCBI Synonym Full Names: | cyclin-dependent kinase 4 |
| NCBI Official Symbol: | CDK4 |
| NCBI Official Synonym Symbols: | CMM3; PSK-J3 |
| NCBI Protein Information: | cyclin-dependent kinase 4; cell division protein kinase 4 |
| UniProt Protein Name: | Cyclin-dependent kinase 4 |
| UniProt Synonym Protein Names: | Cell division protein kinase 4; PSK-J3 |
| Protein Family: | Cyclin-dependent kinase |
| UniProt Gene Name: | CDK4 |
| UniProt Entry Name: | CDK4_HUMAN |