Anti-CDH12 Antibody (CAB10206)
- SKU:
- CAB10206
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-CDH12 Antibody |
| 抗体コード: | CAB10206 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 390-540 of human CDH12 (NP_004052.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:1000 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-251MG, HepG2, 22Rv1, Mouse brain, Mouse liver, Rat brain, Rat pancreas, Rat liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 390-540 of human CDH12 (NP_004052.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | TMEV YEDT PVGT IIGA VTAQ DLDV GSSA VRYF IDWK SDGD SYFT IDGN EGTI ATNE LLDR ESTA QYNF SIIA SKVS NPLL TSKV NILI NVLD VNEF PPEI SVPY ETAV CENA KPGQ IIQI VSAA DRDL SPAG QQFS FRLS PEAA IKPN FTV |
| 遺伝子ID: | 1010 |
| Uniprot: | P55289 |
| セルラーロケーション: | Cell membrane, Single-pass type I membrane protein |
| 計算された分子量: | 84kDa/88kDa |
| 観察された分子量: | 110kDa |
| 同義語: | CDH12, CDHB |
| バックグラウンド: | This gene encodes a type II classical cadherin of the cadherin superfamily. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature cadherin protein. These integral membrane proteins mediate calcium-dependent cell-cell adhesion and are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a histidine-alanine-valine (HAV) cell adhesion recognition sequence specific to type I cadherins. This particular cadherin appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis. |
| UniProt Protein Function: | CDH12: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 5p14.3 Cellular Component: integral to membrane; plasma membrane Molecular Function:calcium ion binding Biological Process: intercellular junction assembly and maintenance; homophilic cell adhesion |
| NCBI Summary: | This gene encodes a type II classical cadherin from the cadherin superfamily of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P55289 |
| NCBI GenInfo Identifier: | 158937438 |
| NCBI Gene ID: | 1010 |
| NCBI Accession: | P55289.2 |
| UniProt Secondary Accession: | P55289,Q86UD2, B2RBT1, |
| UniProt Related Accession: | P55289 |
| Molecular Weight: | 88,332 Da |
| NCBI Full Name: | Cadherin-12 |
| NCBI Synonym Full Names: | cadherin 12, type 2 (N-cadherin 2) |
| NCBI Official Symbol: | CDH12 |
| NCBI Official Synonym Symbols: | CDHB |
| NCBI Protein Information: | cadherin-12; Br-cadherin; N-cadherin 2; brain cadherin; brain-cadherin; neuronal cadherin 2; neural type cadherin 2; cadherin, neural type, 2 |
| UniProt Protein Name: | Cadherin-12 |
| UniProt Synonym Protein Names: | Brain cadherin; BR-cadherin; Neural type cadherin 2; N-cadherin 2 |
| Protein Family: | Cadherin |
| UniProt Gene Name: | CDH12 |
| UniProt Entry Name: | CAD12_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CDH12 antibody (CAB10206) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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