Anti-CDC25C Antibody (RACO0171)
- SKU:
- RACO0171
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | CDC25C Antibody |
| Product SKU: | RACO0171 |
| サイズ: | 50ul |
| 宿主種: | Human |
| 申し込み: | ELISA, WB, IHC, IF, IP |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human CDC25C |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity. |
| シノニム: | M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C, CDC25C |
 | IHC image of RACO0171 diluted at 1:165 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with RACO0171 at 1:55, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
 | Western Blot. Positive WB detected in Hela whole cell lysate K562 whole cell lysate Raji whole cell lysate 293 whole cell lysate All lanes Cdc25C antibody at 1.65µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 54, 52, 49, 46 KDa. Observed band size: 60 KDa. |
| UniProt Protein Function: | CDC25C: a member of the MPI phosphatase family. Activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. Shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. At least four transcript variants for this gene exist. Three splice variant isoforms have been described. |
| UniProt Protein Details: | Protein type:EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity Chromosomal Location of Human Ortholog: 5q31 Cellular Component: cytosol; nucleoplasm; nucleus Molecular Function:phosphoprotein phosphatase activity; protein binding; protein kinase binding; WW domain binding Biological Process: cell proliferation; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA replication; G2/M transition of mitotic cell cycle; regulation of cell cycle; regulation of cyclin-dependent protein kinase activity; regulation of mitosis |
| NCBI Summary: | This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015] |
| UniProt Code: | P30307 |
| NCBI GenInfo Identifier: | 116242631 |
| NCBI Gene ID: | 995 |
| NCBI Accession: | P30307.2 |
| UniProt Secondary Accession: | P30307,Q96PL3, Q9H168, Q9H2E8, Q9H2E9, Q9H2F1, D3DQB8 |
| UniProt Related Accession: | P30307 |
| Molecular Weight: | 45,608 Da |
| NCBI Full Name: | M-phase inducer phosphatase 3 |
| NCBI Synonym Full Names: | cell division cycle 25C |
| NCBI Official Symbol: | CDC25C |
| NCBI Official Synonym Symbols: | CDC25; PPP1R60 |
| NCBI Protein Information: | M-phase inducer phosphatase 3 |
| UniProt Protein Name: | M-phase inducer phosphatase 3 |
| UniProt Synonym Protein Names: | Dual specificity phosphatase Cdc25C |
| UniProt Gene Name: | CDC25C |
| UniProt Entry Name: | MPIP3_HUMAN |