Anti-CD274 Antibody (RACO0526)
- SKU:
- RACO0526
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | CD274 Antibody |
| Product SKU: | RACO0526 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human PD-L1 (CD274) |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. |
| シノニム: | Programmed cell death 1 ligand 1 (PD-L1) (PDCD1 ligand 1) (Programmed death ligand 1) (B7 homolog 1) (B7-H1) (CD antigen CD274), CD274, B7H1 PDCD1L1 PDCD1LG1 PDL1 |
 | IHC image of RACO0526 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | Western Blot. Positive WB detected in HepG2 whole cell lysate K562 whole cell lysate A549 whole cell lysate Hela whole cell lysate All lanes CD274 Antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 34, 21, 21 kDa. Observed band size: 45 kDa. |
| UniProt Protein Function: | CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 9p24.1 Cellular Component: plasma membrane Molecular Function:protein binding Biological Process: cell surface receptor linked signal transduction; immune response; negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; negative regulation of interleukin-10 production; positive regulation of T cell proliferation; regulation of activated T cell proliferation; response to cytokine stimulus; signal transduction; T cell costimulation |
| NCBI Summary: | This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| UniProt Code: | Q9NZQ7 |
| NCBI GenInfo Identifier: | 83287884 |
| NCBI Gene ID: | 29126 |
| NCBI Accession: | Q9NZQ7.1 |
| UniProt Secondary Accession: | Q9NZQ7,Q14CJ2, Q2V8D5, Q66RK1, Q6WEX4, Q9NUZ5, B2RBA2 B4DU27, |
| UniProt Related Accession: | Q9NZQ7 |
| Molecular Weight: | 20,454 Da |
| NCBI Full Name: | Programmed cell death 1 ligand 1 |
| NCBI Synonym Full Names: | CD274 molecule |
| NCBI Official Symbol: | CD274 |
| NCBI Official Synonym Symbols: | B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1 |
| NCBI Protein Information: | programmed cell death 1 ligand 1 |
| UniProt Protein Name: | Programmed cell death 1 ligand 1 |
| UniProt Synonym Protein Names: | B7 homolog 1; B7-H1; CD_antigen: CD274 |
| UniProt Gene Name: | CD274 |