Anti-CAMKK1 Antibody (CAB16716)
- SKU:
- CAB16716
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-CAMKK1 Antibody |
| 抗体コード: | CAB16716 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 399-520 of human CAMKK1 (NP_757344.2). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | LO2, HeLa, A-549 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 399-520 of human CAMKK1 (NP_757344.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | LHRK IKNE PVVF PEEP EISE ELKD LILK MLDK NPET RIGV PDIK LHPW VTKN GEEP LPSE EEHC SVVE VTEE EVKN SVRL IPSW TTVI LVKS MLRK RSFG NPFE PQAR REER SMSA PGNL LV |
| 遺伝子ID: | 84254 |
| Uniprot: | Q8N5S9 |
| セルラーロケーション: | Cytoplasm, Nucleus |
| 計算された分子量: | 55kDa/57kDa |
| 観察された分子量: | 56kDa |
| 同義語: | CAMKK1, CAMKKA |
| バックグラウンド: | The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene. |
| UniProt Protein Function: | CAMKK1: a calcium/calmodulin-dependent S/T protein kinase of the CAMKK family. Phosphorylates CAMK1, CAMK1D, CAMK1G and CAMK4. Involved in regulating cell apoptosis. Promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death. 2 isoforms of the human protein are produced by alternative splicing. The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition. Binding of calmodulin may releave intrasteric autoinhibition. May be regulated through phosphorylation by CAMK1 and CAMK4. Appears to be autophosphorylated in a Ca2+/calmodulin-dependent manner. Phosphorylation of Ser-458 is blocked upon binding to Ca2+/calmodulin. |
| UniProt Protein Details: | Protein type:Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.17; Other group; CAMKK family; Meta subfamily Chromosomal Location of Human Ortholog: 17p13.2 Cellular Component: cytosol Molecular Function:calmodulin-dependent protein kinase activity; protein binding |
| NCBI Summary: | The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8N5S9 |
| NCBI GenInfo Identifier: | 56404620 |
| NCBI Gene ID: | 84254 |
| NCBI Accession: | Q8N5S9.2 |
| UniProt Secondary Accession: | Q8N5S9,Q9BQH3, |
| UniProt Related Accession: | Q8N5S9 |
| Molecular Weight: | 57,523 Da |
| NCBI Full Name: | Calcium/calmodulin-dependent protein kinase kinase 1 |
| NCBI Synonym Full Names: | calcium/calmodulin dependent protein kinase kinase 1 |
| NCBI Official Symbol: | CAMKK1 |
| NCBI Official Synonym Symbols: | CAMKKA |
| NCBI Protein Information: | calcium/calmodulin-dependent protein kinase kinase 1 |
| UniProt Protein Name: | Calcium/calmodulin-dependent protein kinase kinase 1 |
| UniProt Synonym Protein Names: | CaM-kinase IV kinase; Calcium/calmodulin-dependent protein kinase kinase alpha; CaM-KK alpha; CaM-kinase kinase alpha; CaMKK alpha |
| Protein Family: | Calcium/calmodulin-dependent protein kinase |
| UniProt Gene Name: | CAMKK1 |
| UniProt Entry Name: | KKCC1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CAMKK1 antibody (CAB16716) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded Human lung cancer using CAMKK1 Rabbit pAb (CAB16716) at dilution of 1:100 (40x lens). |
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