Anti-CA2 Antibody (CAB18034)[KO Validated]
![Anti-CA2 Antibody (CAB18034)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/608x608/products/14386/14273/anti-ca2-antibody-cab18034ko-validated__24493__11574.1645802651.jpg?c=1 "Anti-CA2 Antibody (CAB18034)[KO Validated]")
- SKU:
- CAB18034
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
![Anti-CA2 Antibody (CAB18034)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/1280x1280/products/14386/14273/anti-ca2-antibody-cab18034ko-validated__24493__11574.1645802651.jpg?c=1&imbypass=on "Anti-CA2 Antibody (CAB18034)[KO Validated]")
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Description
| 抗体名: | Anti-CA2 Antibody [KO Validated] |
| 抗体コード: | CAB18034 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human CA2 (NP_000058.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | 293T |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human CA2 (NP_000058.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MSHH WGYG KHNG PEHW HKDF PIAK GERQ SPVD IDTH TAKY DPSL KPLS VSYD QATS LRIL NNGH AFNV EFDD SQDK AVLK GGPL DGTY RLIQ FHFH WGSL DGQG SEHT VDKK KYAA ELHL VHWN TKYG DFGK AVQQ PDGL AVLG IFLK VGSA KPGL QKVV DVLD SIKT KGKS ADFT NFDP RGLL PESL DYWT YPGS LTTP PLLE CVTW IVLK EPIS VSSE QVLK FRKL NFNG EGEP EELM VDNW RPAQ PL |
| 遺伝子ID: | 760 |
| Uniprot: | P00918 |
| セルラーロケーション: | Cell membrane, Cytoplasm |
| 計算された分子量: | 29kDa |
| 観察された分子量: | 28kDa |
![Anti-CA2 Antibody [KO Validated] (CAB18034)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/v/554/A18034_1__27651.jpg) | Western blot analysis of extracts from normal (control) and CA2 knockout (KO) 293T cells, using CA2 antibody (CAB18034) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
| 同義語: | CA2, CA-II, CAC, CAII, Car2, HEL-76, HEL-S-282 |
| バックグラウンド: | The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | CA2: Essential for bone resorption and osteoclast differentiation. Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Interacts with SLC4A4. Interaction with SLC4A7 regulates SLC4A7 transporter activity. Activated by X-ray, histamine, L-adrenaline, L- and D-phenylalanine, L- and D-histidine, L-His-OMe and beta-Ala- His (carnosine). Competitively inhibited by saccharin, thioxolone, coumarins, 667-coumate, celecoxib (Celebrex), valdecoxib (Bextra), SC-125, SC-560, diclofenac, acetate, azide, bromide, sulfonamide derivatives such as acetazolamide (AZA), methazolamide (MZA), ethoxzolamide (EZA), dichlorophenamide (DCP), brinzolamide, dansylamide, thiabendazole-5-sulfonamide, trifluoromethane sulfonamide and N-hydroxysulfamide, fructose-based sugar sulfamate RWJ-37497, and Foscarnet (phosphonoformate trisodium salt). Repressed strongly by hydrogen sulfide(HS) and weakly by nitrate (NO(3)). Esterase activity weakly reduced by cyanamide. N- hydroxyurea interfers with zinc binding and inhibit activity. Belongs to the alpha-carbonic anhydrase family. |
| UniProt Protein Details: | Protein type:Lyase; EC 4.2.1.1; Energy Metabolism - nitrogen Chromosomal Location of Human Ortholog: 8q22 Cellular Component: extracellular space; microvillus; apical part of cell; axon; basolateral plasma membrane; cytoplasm; plasma membrane; cytosol; myelin sheath Molecular Function:protein binding; carbonate dehydratase activity; zinc ion binding Biological Process: secretion; positive regulation of osteoclast differentiation; positive regulation of synaptic transmission, GABAergic; positive regulation of cellular pH reduction; one-carbon compound metabolic process; odontogenesis of dentine-containing teeth; response to zinc ion; bicarbonate transport; response to estrogen stimulus; regulation of intracellular pH; positive regulation of bone resorption; morphogenesis of an epithelium; kidney development; response to pH Disease: Osteopetrosis, Autosomal Recessive 3 |
| NCBI Summary: | The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014] |
| UniProt Code: | P00918 |
| NCBI GenInfo Identifier: | 115456 |
| NCBI Gene ID: | 760 |
| NCBI Accession: | P00918.2 |
| UniProt Secondary Accession: | P00918,Q6FI12, Q96ET9, B2R7G8, |
| UniProt Related Accession: | P00918 |
| Molecular Weight: | 260 |
| NCBI Full Name: | Carbonic anhydrase 2 |
| NCBI Synonym Full Names: | carbonic anhydrase II |
| NCBI Official Symbol: | CA2 |
| NCBI Official Synonym Symbols: | CAC; CAII; Car2; CA-II; HEL-76 |
| NCBI Protein Information: | carbonic anhydrase 2; carbonic anhydrase B; carbonic anhydrase C; carbonic dehydratase; carbonate dehydratase II; epididymis luminal protein 76 |
| UniProt Protein Name: | Carbonic anhydrase 2 |
| UniProt Synonym Protein Names: | Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II |
| Protein Family: | Beta-carotene hydroxylase |
| UniProt Gene Name: | CA2 |
| UniProt Entry Name: | CAH2_HUMAN |