Anti-BTK Antibody (RACO0551)
- SKU:
- RACO0551
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Description
| 商品名: | BTK Antibody |
| Product SKU: | RACO0551 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, IHC |
| 推奨される希釈: | IHC:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human BTK |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis. |
| シノニム: | Tyrosine-protein kinase BTK (EC 2.7.10.2) (Agammaglobulinemia tyrosine kinase) (ATK) (B-cell progenitor kinase) (BPK) (Bruton tyrosine kinase), BTK, AGMX1 ATK BPK |
 | IHC image of RACO0551 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| UniProt Protein Function: | Btk: a tyrosine kinase of the Tec family. Plays a crucial role in B-cell ontogeny. Defects cause X-linked agammaglobulinemia, an immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. Truncated splice forms found in childhood leukemias may underlie radiation resistance of tumors through inhibition of apoptosis. |
| UniProt Protein Details: | Protein type:Kinase, protein; Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Tec family Cellular Component: cytoplasm; cytoplasmic vesicle; cytosol; extrinsic to internal side of plasma membrane; intracellular membrane-bound organelle; lipid raft; mast cell granule; membrane; nucleus; perinuclear region of cytoplasm; plasma membrane Molecular Function:ATP binding; identical protein binding; kinase activity; lipid binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; phosphatidylinositol-3,4,5-triphosphate binding; protein binding; protein kinase activity; protein-tyrosine kinase activity; transferase activity Biological Process: adaptive immune response; apoptosis; cell maturation; histamine secretion by mast cell; I-kappaB kinase/NF-kappaB cascade; immune system process; innate immune response; negative regulation of cytokine production; peptidyl-tyrosine phosphorylation; phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cell proliferation; regulation of transcription, DNA-dependent; response to organic substance; transcription, DNA-dependent; transmembrane receptor protein tyrosine kinase signaling pathway |
| NCBI Summary: | The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013] |
| UniProt Code: | Q06187 |
| NCBI GenInfo Identifier: | 547759 |
| NCBI Gene ID: | 695 |
| NCBI Accession: | Q06187.3 |
| UniProt Secondary Accession: | Q06187,Q61365, |
| UniProt Related Accession: | Q06187 |
| Molecular Weight: | |
| NCBI Full Name: | Tyrosine-protein kinase BTK |
| NCBI Synonym Full Names: | Bruton tyrosine kinase |
| NCBI Official Symbol: | BTK |
| NCBI Official Synonym Symbols: | AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1 |
| NCBI Protein Information: | tyrosine-protein kinase BTK |
| UniProt Protein Name: | Tyrosine-protein kinase BTK |
| UniProt Synonym Protein Names: | Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase; Kinase EMB |
| Protein Family: | Tyrosine-protein kinase |
| UniProt Gene Name: | Btk |
| UniProt Entry Name: | BTK_MOUSE |