Anti-BGLAP Antibody (CAB6205)
- SKU:
- CAB6205
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-BGLAP Antibody |
| 抗体コード: | CAB6205 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BGLAP (NP_954642.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | U-87MG, Rat lung |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BGLAP (NP_954642.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MRAL TLLA LLAL AALC IAGQ AGAK PSGA ESSK GAAF VSKQ EGSE VVKR PRRY LYQW LGAP VPYP DPLE PRRE VCEL NPDC DELA DHIG FQEA YRRF YGPV |
| 遺伝子ID: | 632 |
| Uniprot: | P02818 |
| セルラーロケーション: | Secreted |
| 計算された分子量: | 10kDa |
| 観察された分子量: | 12KDa |
| 同義語: | BGLAP, BGP, OC, OCN, osteocalcin |
| バックグラウンド: | This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. |
| UniProt Protein Function: | osteocalcin: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Belongs to the osteocalcin/matrix Gla protein family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted; Cell development/differentiation Chromosomal Location of Human Ortholog: 1q22 Cellular Component: Golgi apparatus; extracellular space; rough endoplasmic reticulum; dendrite; cytoplasm; perikaryon Molecular Function:structural constituent of bone; hydroxyapatite binding; calcium ion binding; structural molecule activity Biological Process: response to drug; response to gravity; regulation of bone resorption; response to glucocorticoid stimulus; cell aging; response to testosterone stimulus; bone mineralization; osteoblast development; osteoblast differentiation; odontogenesis; response to vitamin D; regulation of osteoclast differentiation; response to ethanol; response to vitamin K; response to zinc ion; response to mechanical stimulus; response to estrogen stimulus; response to hydroxyisoflavone; response to activity; cell adhesion; skeletal development; regulation of bone mineralization |
| NCBI Summary: | This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015] |
| UniProt Code: | P02818 |
| NCBI GenInfo Identifier: | 129253 |
| NCBI Gene ID: | 632 |
| NCBI Accession: | P02818.2 |
| UniProt Secondary Accession: | P02818,Q5TCK6, |
| UniProt Related Accession: | P02818 |
| Molecular Weight: | 10,963 Da |
| NCBI Full Name: | Osteocalcin |
| NCBI Synonym Full Names: | bone gamma-carboxyglutamate (gla) protein |
| NCBI Official Symbol: | BGLAP |
| NCBI Official Synonym Symbols: | OC; BGP; OCN |
| NCBI Protein Information: | osteocalcin; bone Gla protein; gamma-carboxyglutamic acid-containing protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin) |
| UniProt Protein Name: | Osteocalcin |
| UniProt Synonym Protein Names: | Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein |
| Protein Family: | Osteocalcin |
| UniProt Gene Name: | BGLAP |
| UniProt Entry Name: | OSTCN_HUMAN |
 | Western blot analysis of extracts of U-87MG cells, using BGLAP antibody (CAB6205) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of extracts of Rat lung, using BGLAP antibody (CAB6205) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunofluorescence analysis of C6 cells using Osteocalcin antibody (CAB6205) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using Osteocalcin antibody (CAB6205) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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