Anti-Beta-adducin Antibody (CAB9025)
- SKU:
- CAB9025
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-Beta-adducin Antibody |
| 抗体コード: | CAB9025 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 580-690 of human ADD2 (NP_001171983.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:1000 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | U-87MG, 293T, K-562, LO2, HT-1080, A-549, Mouse heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 580-690 of human ADD2 (NP_001171983.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | DGEK ETAP EEPG SPAK SAPA SPVQ SPAK EAET KSPL VSPS KSLE EGTK KTET SKAA TTEP ETTQ PEGV VVNG REEE QTAE EILS KGLS QMTT SADT DVDT SKDK TESV TSG |
| 遺伝子ID: | 119 |
| Uniprot: | P35612 |
| セルラーロケーション: | Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cytoskeleton |
| 計算された分子量: | 11kDa/31kDa/62kDa/64kDa/66kDa/72kDa/80kDa |
| 観察された分子量: | 81kDa |
| 同義語: | ADD2, ADDB, adducin 2 |
| バックグラウンド: | Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described. |
| UniProt Protein Function: | ADD2: a cytoskeletal protein that promotes the assembly of the spectrin-actin network. Adducin is a heterodimeric protein that consists of related subunits. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Beta-adducin is restricted to brain and hematopoietic tissues. Three alternatively-spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 2p13.3 Cellular Component: cytoplasmic membrane-bound vesicle; cytosol; F-actin capping protein complex; plasma membrane Molecular Function:actin binding; actin filament binding; calmodulin binding; protein heterodimerization activity; protein homodimerization activity; spectrin binding Biological Process: actin cytoskeleton organization and biogenesis; actin filament bundle formation; barbed-end actin filament capping; ion transport; positive regulation of protein binding; protein complex assembly; transmembrane transport |
| NCBI Summary: | Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jun 2010] |
| UniProt Code: | P35612 |
| NCBI GenInfo Identifier: | 543774 |
| NCBI Gene ID: | 119 |
| NCBI Accession: | P35612.3 |
| UniProt Secondary Accession: | P35612,Q13482, Q16412, Q59G82, Q5U5P4, Q6P0P2, Q6PGQ4 Q7Z688, A8K4P2, B4DM17, D6W5G7, D6W5G8, |
| UniProt Related Accession: | P35612 |
| Molecular Weight: | 64,217 Da |
| NCBI Full Name: | Beta-adducin |
| NCBI Synonym Full Names: | adducin 2 |
| NCBI Official Symbol: | ADD2 |
| NCBI Official Synonym Symbols: | ADDB |
| NCBI Protein Information: | beta-adducin |
| UniProt Protein Name: | Beta-adducin |
| UniProt Synonym Protein Names: | Erythrocyte adducin subunit beta |
| Protein Family: | Beta-adducin |
| UniProt Gene Name: | ADD2 |
| UniProt Entry Name: | ADDB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ADD2 antibody (CAB9025) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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