Anti-BDKRB1 Antibody (CAB1959)
- SKU:
- CAB1959
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-BDKRB1 Antibody |
| 抗体コード: | CAB1959 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BDKRB1 (NP_000701.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | A-549, A-431 |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BDKRB1 (NP_000701.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MASS WPPL ELQS SNQS QLFP QNAT ACDN APEA WDLL HRVL PTFI ISIC FFGL LGNL FVLL VFLL PRRQ LNVA EIYL ANLA ASDL VFVL GLPF WAEN IWNQ |
| 遺伝子ID: | 623 |
| Uniprot: | P46663 |
| セルラーロケーション: | Cell membrane, Multi-pass membrane protein |
| 計算された分子量: | 40kDa |
| 観察された分子量: | 40kDa |
| 同義語: | BDKRB1, B1BKR, B1R, BDKRB2, BKB1R, BKR1, BRADYB1 |
| バックグラウンド: | Bradykinin, a 9 aa peptide, is generated in pathophysiologic conditions such as inflammation, trauma, burns, shock, and allergy. Two types of G-protein coupled receptors have been found which bind bradykinin and mediate responses to these pathophysiologic conditions. The protein encoded by this gene is one of these receptors and is synthesized de novo following tissue injury. Receptor binding leads to an increase in the cytosolic calcium ion concentration, ultimately resulting in chronic and acute inflammatory responses. Several transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | BKR1: This is a receptor for bradykinin. Could be a factor in chronic pain and inflammation. Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB1 sub-subfamily. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1 Chromosomal Location of Human Ortholog: 14q32.1-q32.2 Cellular Component: endoplasmic reticulum; integral to plasma membrane; plasma membrane Molecular Function:protein binding Biological Process: elevation of cytosolic calcium ion concentration |
| NCBI Summary: | Bradykinin, a 9 aa peptide, is generated in pathophysiologic conditions such as inflammation, trauma, burns, shock, and allergy. Two types of G-protein coupled receptors have been found which bind bradykinin and mediate responses to these pathophysiologic conditions. The protein encoded by this gene is one of these receptors and is synthesized de novo following tissue injury. Receptor binding leads to an increase in the cytosolic calcium ion concentration, ultimately resulting in chronic and acute inflammatory responses. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| UniProt Code: | P46663 |
| NCBI GenInfo Identifier: | 85681914 |
| NCBI Gene ID: | 623 |
| NCBI Accession: | P46663.3 |
| UniProt Secondary Accession: | P46663,Q546S7, Q8N0Y8, A8K7F5, |
| UniProt Related Accession: | P46663 |
| Molecular Weight: | 40,495 Da |
| NCBI Full Name: | B1 bradykinin receptor |
| NCBI Synonym Full Names: | bradykinin receptor B1 |
| NCBI Official Symbol: | BDKRB1 |
| NCBI Official Synonym Symbols: | B1R; BKR1; B1BKR; BKB1R; BDKRB2; BRADYB1 |
| NCBI Protein Information: | B1 bradykinin receptor |
| UniProt Protein Name: | B1 bradykinin receptor |
| Protein Family: | B1 bradykinin receptor |
| UniProt Gene Name: | BDKRB1 |
| UniProt Entry Name: | BKRB1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BDKRB1 antibody (CAB1959) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of A549 cells using BDKRB1 Rabbit pAb (CAB1959) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of C6 cells using BDKRB1 Rabbit pAb (CAB1959) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using BDKRB1 Rabbit pAb (CAB1959) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining. |
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