Anti-BCLAF1 Antibody (CAB16473)
- SKU:
- CAB16473
- Product type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-BCLAF1 Antibody |
| 抗体コード: | CAB16473 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of human BCLAF1 (NP_001070909.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Mouse, Rat |
| ポジティブサンプル: | Mouse brain, Rat brain |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of human BCLAF1 (NP_001070909.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | REEE FKTH HEMK EYSG FAGV SRPR GTFF RIRG RGRA RGVF AGTN TGPN NSNT TFQK RPKE EEWD PEYT PKSK KYFL HDDR DDGV DYWA KRGR GRGT FQRG RGRF NFKK SGSS PKWT HDKY QGDG IVED EEET MENN EEKK DRRK EEKE |
| 遺伝子ID: | 9774 |
| Uniprot: | Q9NYF8 |
| セルラーロケーション: | Cytoplasm, Nucleus, Nucleus speckle, nucleoplasm |
| 計算された分子量: | 85kDa/100kDa/105kDa/106kDa |
| 観察された分子量: | 145kDa |
| 同義語: | BCLAF1, BTF, bK211L9.1 |
| バックグラウンド: | This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | BCLAF1: a ubiquitous death-promoting transcriptional repressor. Interacts with Bcl-2 related proteins, lamin A, emerin, the adenovirus E1B 19 kDa protein and DNA. An emerin mutant S54F, which binds normally to barrier-to-autointegration factor (BAF), lamin A and GCL, selectively disrupts its binding to BCLAF1. BCLAF1 is found in dot-like structures throughout the nuclear interior in HeLa cells. Proapoptotic stimuli induce its redistribution to a distinct zone near the nuclear envelope. Four alternatively spliced human isoforms have been described. |
| UniProt Protein Details: | Protein type:Transcription factor; RNA-binding Chromosomal Location of Human Ortholog: 6q23.3 Cellular Component: nuclear speck; nucleolus; nucleoplasm; nucleus Molecular Function:DNA binding; protein binding Biological Process: apoptosis; negative regulation of transcription, DNA-dependent; positive regulation of apoptosis; regulation of transcription in response to stress |
| NCBI Summary: | This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NYF8 |
| NCBI GenInfo Identifier: | 47605556 |
| NCBI Gene ID: | 9774 |
| NCBI Accession: | Q9NYF8.2 |
| UniProt Secondary Accession: | Q9NYF8,Q14673, Q86WU6, Q86WY0, A2RU75, B7ZM58, E1P586 |
| UniProt Related Accession: | Q9NYF8 |
| Molecular Weight: | 85,937 Da |
| NCBI Full Name: | Bcl-2-associated transcription factor 1 |
| NCBI Synonym Full Names: | BCL2 associated transcription factor 1 |
| NCBI Official Symbol: | BCLAF1 |
| NCBI Official Synonym Symbols: | BTF; bK211L9.1 |
| NCBI Protein Information: | bcl-2-associated transcription factor 1 |
| UniProt Protein Name: | Bcl-2-associated transcription factor 1 |
| Protein Family: | Bcl-2-associated transcription factor |
| UniProt Gene Name: | BCLAF1 |
| UniProt Entry Name: | BCLF1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BCLAF1 antibody (CAB16473) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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