Anti-ATF5 Antibody (RACO0278)
- SKU:
- RACO0278
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | ATF5 Antibody |
| Product SKU: | RACO0278 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200 |
| 反応性: | Human, Rat |
| 免疫原: | A synthesized peptide derived from human ATF5 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation (PubMed:10373550, PubMed:15358120, PubMed:21212266, PubMed:20654631). Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4 (PubMed:15358120). Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate (By similarity). Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation (By similarity). Critical for survival of mature olfactory sensory neurons (OSN), directs expression of OSN-specific genes (By similarity). May be involved in osteogenic differentiation (PubMed:22442021). Promotes cell proliferation and survival by inducing the expression of EGR1 sinergistically with ELK1. Once acetylated by EP300, binds to ARE sequences on target genes promoters, such as BCL2 and EGR1 (PubMed:21791614). Plays an anti-apoptotic role through the transcriptional regulation of BCL2, this function seems to be cell type-dependent (By similarity). Cooperates with NR1I3/CAR in the transcriptional activation of CYP2B6 in liver (PubMed:18332083). In hepatic cells, represses CRE-dependent transcription and inhibits proliferation by blocking at G2/M phase (PubMed:22528486, PubMed:18701499). May act as a negative regulator of IL1B transduction pathway in liver (PubMed:24379400). Upon IL1B stimulus, cooperates with NLK to activate the transactivation activity of C/EBP subfamily members (PubMed:25512613). Besides its function of transcription factor, acts as a cofactor of CEBPB to activate CEBPA and promote adipocyte differentiation (PubMed:24216764). Regulates centrosome dynamics in a cell-cycle- and centriole-age-dependent manner. Forms 9-foci symmetrical ring scaffold around the mother centriole to control centrosome function and the interaction between centrioles and pericentriolar material (PubMed:26213385). |
| シノニム: | Cyclic AMP-dependent transcription factor ATF-5 (cAMP-dependent transcription factor ATF-5) (Activating transcription factor 5) (Transcription factor ATFx), ATF5, ATFX |
 | IHC image of RACO0278 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | Western Blot. Positive WB detected in Hela whole cell lysate 293 whole cell lysate Jurkat whole cell lysate SH-SY5Y whole cell lysate THP-1 whole cell lysate PC-3 whole cell lysate Rat brain tissue. All lanes ATF5 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 31 kDa. Observed band size: 35 kDa. |
| UniProt Protein Function: | ATF-5: Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Belongs to the bZIP family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 19q13.3 Cellular Component: centrosome; cytoplasm; nucleoplasm; nucleus Molecular Function:chromatin binding; kinase binding; protein binding; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity; tubulin binding Biological Process: fat cell differentiation; granule cell precursor proliferation; negative regulation of apoptosis; negative regulation of cell proliferation; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of centrosome cycle; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent |
| UniProt Code: | Q9Y2D1 |
| NCBI GenInfo Identifier: | 308153647 |
| NCBI Gene ID: | 22809 |
| NCBI Accession: | Q9Y2D1.4 |
| UniProt Secondary Accession: | Q9Y2D1,Q9BSA1, Q9UNQ3, B3KND3, |
| UniProt Related Accession: | Q9Y2D1 |
| Molecular Weight: | 30,674 Da |
| NCBI Full Name: | Cyclic AMP-dependent transcription factor ATF-5 |
| NCBI Synonym Full Names: | activating transcription factor 5 |
| NCBI Official Symbol: | ATF5 |
| NCBI Official Synonym Symbols: | ATFX; HMFN0395 |
| NCBI Protein Information: | cyclic AMP-dependent transcription factor ATF-5 |
| UniProt Protein Name: | Cyclic AMP-dependent transcription factor ATF-5 |
| UniProt Synonym Protein Names: | Activating transcription factor 5; Transcription factor ATFx |
| Protein Family: | Cyclic AMP-dependent transcription factor |
| UniProt Gene Name: | ATF5 |
| UniProt Entry Name: | ATF5_HUMAN |