Anti-ATF4 Antibody (RACO0178)
- SKU:
- RACO0178
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Application:
- FC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | ATF4 Antibody |
| Product SKU: | RACO0178 |
| サイズ: | 50ul |
| 宿主種: | Human |
| 申し込み: | ELISA, WB, IHC, IF, FC, IP |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human ATF4 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production (By similarity). It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. Regulates the induction of DDIT3/CHOP and asparagine synthetase (ASNS) in response to endoplasmic reticulum (ER) stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes. During ER stress response, activates the transcription of NLRP1, possibly in concert with other factors (PubMed:26086088). |
| シノニム: | Cyclic AMP-dependent transcription factor ATF-4, cAMP-dependent transcription factor ATF-4, Activating transcription factor 4, Cyclic AMP-responsive element-binding protein 2, CREB-2, cAMP-responsive element-binding protein 2, DNA-binding protein TAXREB67, Tax-responsive enhancer element-binding protein 67, TaxREB67, ATF4, CREB2, TXREB |
 | IHC image of RACO0178 diluted at 1:160 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of HepG2 cells with RACO0178 at 1:53, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
 | Western Blot. Positive WB detected in Hela whole cell lysate MCF-7 whole cell lysate 293 whole cell lysate HepG2 whole cell lysate Jurkat whole cell lysate K562 whole cell lysate All lanes ATF4 antibody at 1.6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 39 KDa. Observed band size: 50 KDa. |
| UniProt Protein Function: | ATF-4: a transcription factor that is a member of the leucine zipper family. Isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). Involved in cisplatin resistance. Other members of the family include AP-1 transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. Two alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: nucleoplasm; neuron projection; cytoplasm; microtubule organizing center; nucleus Molecular Function:protein C-terminus binding; leucine zipper domain binding; protein binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; transcription factor activity Biological Process: amino acid metabolic process; transcription from RNA polymerase II promoter; positive regulation of apoptosis; unfolded protein response; positive regulation of transcription, DNA-dependent; negative regulation of potassium ion transport; positive regulation of transcription from RNA polymerase I promoter; gluconeogenesis; cellular response to glucose starvation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; gamma-aminobutyric acid signaling pathway |
| NCBI Summary: | This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011] |
| UniProt Code: | P18848 |
| NCBI GenInfo Identifier: | 116241262 |
| NCBI Gene ID: | 468 |
| NCBI Accession: | P18848.3 |
| UniProt Secondary Accession: | P18848,Q9UH31, |
| UniProt Related Accession: | P18848 |
| Molecular Weight: | Calculated Molecular Weight: 38kDaObserved Molecular Weight: 38kDa |
| NCBI Full Name: | Cyclic AMP-dependent transcription factor ATF-4 |
| NCBI Synonym Full Names: | activating transcription factor 4 |
| NCBI Official Symbol: | ATF4 |
| NCBI Official Synonym Symbols: | CREB2; TXREB; CREB-2; TAXREB67 |
| NCBI Protein Information: | cyclic AMP-dependent transcription factor ATF-4; DNA-binding protein TAXREB67; tax-responsive enhancer element B67; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; cyclic AMP-responsive element-binding protein 2; tax-responsive enhancer element-binding protein 67 |
| UniProt Protein Name: | Cyclic AMP-dependent transcription factor ATF-4 |
| UniProt Synonym Protein Names: | Activating transcription factor 4; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; Tax-responsive enhancer element-binding protein 67; TaxREB67 |
| Protein Family: | Cyclic AMP-dependent transcription factor |
| UniProt Gene Name: | ATF4 |
| UniProt Entry Name: | ATF4_HUMAN |