Anti-APC Antibody (RACO0248)
- SKU:
- RACO0248
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | APC Antibody |
| Product SKU: | RACO0248 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200 |
| 反応性: | Human, Rat |
| 免疫原: | A synthesized peptide derived from human APC |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization. |
| シノニム: | Adenomatous polyposis coli protein (Protein APC) (Deleted in polyposis 2.5), APC, DP2.5 |
 | IHC image of RACO0248 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | Western Blot. Positive WB detected in Hela whole cell lysate K562 whole cell lysate SH-SY5Y whole cell lysate A549 whole cell lysate Rat Brain whole cell lysate All lanes APC antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 312, 301, 309 kDa. Observed band size: 160 kDa. |
| UniProt Code: | P25054 |
| NCBI GenInfo Identifier: | 97535708 |
| NCBI Gene ID: | 324 |
| NCBI Accession: | P25054.2 |
| UniProt Secondary Accession: | P25054,Q15162, Q15163, Q93042, D3DT03, |
| UniProt Related Accession: | P25054 |
| Molecular Weight: | 461 |
| NCBI Full Name: | Adenomatous polyposis coli protein |
| NCBI Synonym Full Names: | adenomatous polyposis coli |
| NCBI Official Symbol: | APC |
| NCBI Official Synonym Symbols: | GS; DP2; DP3; BTPS2; DP2.5; PPP1R46 |
| NCBI Protein Information: | adenomatous polyposis coli protein; deleted in polyposis 2.5; adenomatosis polyposis coli tumor suppressor; protein phosphatase 1, regulatory subunit 46 |
| UniProt Protein Name: | Adenomatous polyposis coli protein |
| UniProt Synonym Protein Names: | Deleted in polyposis 2.5 |
| Protein Family: | APC-related protein |
| UniProt Gene Name: | APC |
| UniProt Entry Name: | APC_HUMAN |