Anti-ANGPT2 Antibody (RACO0376)
- SKU:
- RACO0376
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | ANGPT2 Antibody |
| Product SKU: | RACO0376 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, IHC |
| 推奨される希釈: | IHC:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human Angiopoietin 2 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. |
| シノニム: | Angiopoietin-2 (ANG-2), ANGPT2 |
 | IHC image of RACO0376 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | IHC image of RACO0376 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| UniProt Protein Function: | ANGPT2: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Interacts with TEK/TIE2, competing for the same binding site as ANGPT1. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 8p23.1 Cellular Component: extracellular space; cell projection; extracellular region; plasma membrane; nucleus Molecular Function:protein binding; metal ion binding; receptor tyrosine kinase binding; receptor binding Biological Process: Tie receptor signaling pathway; organ regeneration; maternal process involved in pregnancy; signal transduction; negative regulation of blood vessel endothelial cell migration; response to organic cyclic substance; germ cell development; negative regulation of positive chemotaxis; response to radiation; negative regulation of angiogenesis; positive regulation of angiogenesis; response to mechanical stimulus; response to hypoxia; response to glucose stimulus; angiogenesis; response to activity; blood coagulation; leukocyte migration |
| NCBI Summary: | The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O15123 |
| NCBI GenInfo Identifier: | 12229555 |
| NCBI Gene ID: | 285 |
| NCBI Accession: | O15123.1 |
| UniProt Secondary Accession: | O15123,Q9NRR7, Q9P2Y7, A0AV38, A8K205, B7ZLM7, |
| UniProt Related Accession: | O15123 |
| Molecular Weight: | 56,848 Da |
| NCBI Full Name: | Angiopoietin-2 |
| NCBI Synonym Full Names: | angiopoietin 2 |
| NCBI Official Symbol: | ANGPT2 |
| NCBI Official Synonym Symbols: | ANG2; AGPT2 |
| NCBI Protein Information: | angiopoietin-2; ANG-2; Tie2-ligand; angiopoietin-2B; angiopoietin-2a |
| UniProt Protein Name: | Angiopoietin-2 |
| UniProt Gene Name: | ANGPT2 |
| UniProt Entry Name: | ANGP2_HUMAN |