Anti-Ambra1 Antibody (CAB12578)
- SKU:
- CAB12578
- Product type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Frequently bought together:
Description
| 抗体名: | Anti-Ambra1 Antibody |
| 抗体コード: | CAB12578 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 961-1208 of human Ambra1 (NP_060219.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Mouse |
| ポジティブサンプル: | Mouse brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 961-1208 of human Ambra1 (NP_060219.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | QLNE TVFT VHSN SRSS ERPG TSRA TWRT DRDM GLMN AIGL QPRN PATS VTSQ GTQT LALQ LQNA ETQT EREV PEPG TAAS GPGE GEGS EYGA SGED ALSR IQRL MAEG GMTA VVQR EQST TMAS MGGF GNNI IVSH RIHR SSQT GTEP GAAH TSSP QPST SRGL LPEA GQLA ERGL SPRT ASWD QPGT PGRE PTQP TLPS SSPV PIPV SLPS AEGP TLHC ELTN NNHL LDGG SSRG DAAG PRGE PRNR |
| 遺伝子ID: | 55626 |
| Uniprot: | Q9C0C7 |
| セルラーロケーション: | Cytoplasmic vesicle, autophagosome |
| 計算された分子量: | 84kDa/132kDa/135kDa/139kDa/142kDa |
| 観察された分子量: | 175kDa |
| 同義語: | AMBRA1, DCAF3, WDR94, FLJ20294 |
| バックグラウンド: |
| UniProt Protein Function: | AMBRA1: a WD40 protein that regulates autophagy and development of the nervous system. A highly conserved vertebrate protein whose expression is mostly confined to the neuroepithelium during early neurogenesis. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. Interacts with ATG6. Forms a complex with ATG6 and PIK3C3. Its functional inactivation in mouse is lethal in utero (starting from embryonic day 14.5), leading to severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, and excessive apoptosis. Hyperproliferation followed by a wave of caspase-dependent cell death was the earliest detectable abnormality in the developing neuroepithelium. Five isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Autophagy; Vesicle Chromosomal Location of Human Ortholog: 11p11.2 Cellular Component: mitochondrial outer membrane; intracellular membrane-bound organelle; mitochondrion; cytoplasm; autophagic vacuole; phagocytic vesicle; axoneme; cytosol Molecular Function:protein binding; ubiquitin protein ligase binding Biological Process: neural tube development; negative regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase activity; autophagy; negative regulation of neuron apoptosis; cell differentiation |
| UniProt Code: | Q9C0C7 |
| NCBI GenInfo Identifier: | 166215833 |
| NCBI Gene ID: | 55626 |
| NCBI Accession: | Q9C0C7.2 |
| UniProt Secondary Accession: | Q9C0C7,A2AH22, |
| UniProt Related Accession: | Q9C0C7 |
| Molecular Weight: | |
| NCBI Full Name: | Activating molecule in BECN1-regulated autophagy protein 1 |
| NCBI Synonym Full Names: | autophagy and beclin 1 regulator 1 |
| NCBI Official Symbol: | AMBRA1 |
| NCBI Official Synonym Symbols: | DCAF3; WDR94 |
| NCBI Protein Information: | activating molecule in BECN1-regulated autophagy protein 1 |
| UniProt Protein Name: | Activating molecule in BECN1-regulated autophagy protein 1 |
| Protein Family: | Activating molecule in BECN1-regulated autophagy protein |
| UniProt Gene Name: | AMBRA1 |
| UniProt Entry Name: | AMRA1_HUMAN |
 | Western blot analysis of extracts of mouse brain, using Ambra1 Antibody (CAB12578) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
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