Anti-ACTN1 Antibody (CAB1160)
- SKU:
- CAB1160
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Monkey
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ACTN1 Antibody |
| 抗体コード: | CAB1160 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Monkey |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human ACTN1 (NP_001123477.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Monkey |
| ポジティブサンプル: | COS-7, PC-3, U-251MG, A673 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human ACTN1 (NP_001123477.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MDHY DSQQ TNDY MQPE EDWD RDLL LDPA WEKQ QRKT FTAW CNSH LRKA GTQI ENIE EDFR DGLK LMLL LEVI SGER LAKP ERGK MRVH KISN VNKA LDFI ASKG VKLV SIGA EEIV DGNV KMTL GMIW TIIL RFAI QDIS VEET SAKE GLLL WCQR KTAP YKNV NIQN FHIS WKDG LGFC ALIH RHRP ELID YGKL RKDD PLTN LNTA FDVA EKYL DIPK MLDA EDIV GTAR PDEK AIMT YVSS FYHA FSGA QKAE TAAN RICK VLAV NQEN EQLM EDYE KLAS DLLE WIRR TIPW LENR VPEN TMHA MQQK LEDF RDYR RLHK PPKV QE |
| 遺伝子ID: | 87 |
| Uniprot: | P12814 |
| セルラーロケーション: | Cell junction, Cell membrane, Cell projection, Cytoplasm, Z line, cytoskeleton, myofibril, ruffle, sarcomere |
| 計算された分子量: | 102kDa/103kDa/105kDa/107kDa |
| 観察された分子量: | 105kDa |
| 同義語: | ACTN1, BDPLT15 |
| バックグラウンド: | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | ACTN1: a cytoskeletal protein of the spectrin superfamily. An actin-binding protein with multiple roles in different cell types. A nonmuscle isoform structurally similar to the erythroid beta spectrin gene. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 14q24|14q22-q24 Cellular Component: extracellular space; cortical actin cytoskeleton; focal adhesion; extracellular region; dendritic spine; fascia adherens; pseudopodium; cytosol; Z disc; ruffle; cell projection; cytoplasm; plasma membrane; stress fiber; nucleus Molecular Function:actin filament binding; integrin binding; protein domain specific binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; double-stranded RNA binding; calcium ion binding; vinculin binding Biological Process: regulation of apoptosis; actin crosslink formation; focal adhesion formation; platelet activation; actin filament bundle formation; extracellular matrix organization and biogenesis; platelet degranulation; negative regulation of cell motility; blood coagulation Disease: Bleeding Disorder, Platelet-type, 15 |
| NCBI Summary: | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P12814 |
| NCBI GenInfo Identifier: | 46397817 |
| NCBI Gene ID: | 87 |
| NCBI Accession: | P12814.2 |
| UniProt Secondary Accession: | P12814,Q1HE25, Q9BTN1, B3V8S3, B4DHH3, B7TY16, |
| UniProt Related Accession: | P12814 |
| Molecular Weight: | 892 |
| NCBI Full Name: | Alpha-actinin-1 |
| NCBI Synonym Full Names: | actinin, alpha 1 |
| NCBI Official Symbol: | ACTN1 |
| NCBI Official Synonym Symbols: | BDPLT15 |
| NCBI Protein Information: | alpha-actinin-1; actinin 1 smooth muscle; non-muscle alpha-actinin-1; F-actin cross-linking protein; alpha-actinin cytoskeletal isoform |
| UniProt Protein Name: | Alpha-actinin-1 |
| UniProt Synonym Protein Names: | Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1 |
| Protein Family: | Alpha-actinin |
| UniProt Gene Name: | ACTN1 |
| UniProt Entry Name: | ACTN1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ACTN1 antibody (CAB1160) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using ACTN1 antibody (CAB1160) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human esophagus using ACTN1 antibody (CAB1160) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using ACTN1 antibody (CAB1160) at dilution of 1:100 (40x lens). |
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