Anti-ABCE1 Antibody (CAB8146)
- SKU:
- CAB8146
- Product type:
- Antibody
- Application:
- WB
- Application:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | ABCE1 Rabbit Polyclonal Antibody |
| 抗体コード: | CAB8146 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 320-599 of human ABCE1 (NP_002931.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | MCF7, Jurkat, 293T, HeLa, Mouse liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 320-599 of human ABCE1 (NP_002931.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | ENLR FRDA SLVF KVAE TANE EEVK KMCM YKYP GMKK KMGE FELA IVAG EFTD SEIM VMLG ENGT GKTT FIRM LAGR LKPD EGGE VPVL NVSY KPQK ISPK STGS VRQL LHEK IRDA YTHP QFVT DVMK PLQI ENII DQEV QTLS GGEL QRVA LALC LGKP ADVY LIDE PSAY LDSE QRLM AARV VKRF ILHA KKTA FVVE HDFI MATY LADR VIVF DGVP SKNT VANS PQTL LAGM NKFL SQLE ITFR RDPN NYRP RINK LNSI KDVE QKKS GNYF FLDD |
| 遺伝子ID: | 6059 |
| Uniprot: | P61221 |
| セルラーロケーション: | Cytoplasm, Mitochondrion |
| 計算された分子量: | 67kDa |
| 観察された分子量: | 67kDa |
| 同義語: | ABCE1, ABC38, OABP, RLI, RLI1, RNASEL1, RNASELI, RNS4I |
| バックグラウンド: | The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the OABP subfamily. Alternatively referred to as the RNase L inhibitor, this protein functions to block the activity of ribonuclease L. Activation of ribonuclease L leads to inhibition of protein synthesis in the 2-5A/RNase L system, the central pathway for viral interferon action. Two transcript variants encoding the same protein have been found for this gene. |
| UniProt Protein Function: | ABCE1: Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly. Belongs to the ABC transporter superfamily. ABCE family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 4q31 Cellular Component: mitochondrion; membrane; cytoplasm Molecular Function:ATPase activity; iron-sulfur cluster binding; ribonuclease inhibitor activity; ATP binding Biological Process: viral reproduction; response to virus; RNA catabolic process; negative regulation of catalytic activity |
| NCBI Summary: | The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the OABP subfamily. Alternatively referred to as the RNase L inhibitor, this protein functions to block the activity of ribonuclease L. Activation of ribonuclease L leads to inhibition of protein synthesis in the 2-5A/RNase L system, the central pathway for viral interferon action. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P61221 |
| NCBI GenInfo Identifier: | 108773784 |
| NCBI Gene ID: | 6059 |
| NCBI Accession: | NP_001035809.1 |
| UniProt Secondary Accession: | P61221,O88793, Q13181, Q13864, Q6NR76, Q96AL0, Q96B10 Q99K66, |
| UniProt Related Accession: | P61221 |
| Molecular Weight: | Calculated: 67kDaObserved: 67kDa |
| NCBI Full Name: | ATP-binding cassette sub-family E member 1 |
| NCBI Synonym Full Names: | ATP-binding cassette, sub-family E (OABP), member 1 |
| NCBI Official Symbol: | ABCE1 |
| NCBI Official Synonym Symbols: | RLI; OABP; ABC38; RNS4I; RNASEL1; RNASELI |
| NCBI Protein Information: | ATP-binding cassette sub-family E member 1 |
| UniProt Protein Name: | ATP-binding cassette sub-family E member 1 |
| UniProt Synonym Protein Names: | 2'-5'-oligoadenylate-binding protein; HuHP68; RNase L inhibitor; Ribonuclease 4 inhibitor; RNS4I |
| Protein Family: | ABC transporter E family |
| UniProt Gene Name: | ABCE1 |
| UniProt Entry Name: | ABCE1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ABCE1 antibody (CAB8146) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of U2OS cells using ABCE1 antibody (CAB8146) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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