WDR83 Antibody (PACO63203)
- SKU:
- PACO63203
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | WDR83 Antibody (PACO63203) |
抗体コード: | PACO63203 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human WD repeat domain-containing protein 83 protein (64-315AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO63203 diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Immunofluorescence staining of HepG2 cells with PACO63203 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | Molecular scaffold protein for various multimeric protein complexes. Acts as a module in the assembly of a multicomponent scaffold for the ERK pathway, linking ERK responses to specific agonists. At low concentrations it enhances ERK activation, whereas high concentrations lead to the inhibition of ERK activation. Also involved in response to hypoxia by acting as a negative regulator of HIF1A/HIF-1-α via its interaction with EGLN3/PHD3. May promote degradation of HIF1A. May act by recruiting signaling complexes to a specific upstream activator (By similarity). May also be involved in pre-mRNA splicing. |
シノニム: | WD repeat domain-containing protein 83 (Mitogen-activated protein kinase organizer 1) (MAPK organizer 1), WDR83, MORG1 |
UniProt Protein Function: | MORG1: Molecular scaffold protein for various multimeric protein complexes. Acts as a module in the assembly of a multicomponent scaffold for the ERK pathway, linking ERK responses to specific agonists. At low concentrations it enhances ERK activation, whereas high concentrations lead to the inhibition of ERK activation. Also involved in response to hypoxia by acting as a negative regulator of HIF1A/HIF-1-alpha via its interaction with EGLN3/PHD3. May promote degradation of HIF1A. May act by recruiting signaling complexes to a specific upstream activator. May also be involved in pre-mRNA splicing. Belongs to the WD repeat MORG1 family. |
UniProt Protein Details: | Protein type:Spliceosome; RNA processing Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: spliceosome; cytoplasm Molecular Function:protein binding Biological Process: RNA splicing, via transesterification reactions; nuclear mRNA splicing, via spliceosome |
NCBI Summary: | This gene encodes a member of the WD-40 protein family. The protein is proposed to function as a molecular scaffold for various multimeric protein complexes. The protein associates with several components of the extracellular signal-regulated kinase (ERK) pathway, and promotes ERK activity in response to serum or other signals. The protein also interacts with egl nine homolog 3 (EGLN3, also known as PHD3) and regulates expression of hypoxia-inducible factor 1, and has been purified as part of the spliceosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009] |
UniProt Code: | Q9BRX9 |
NCBI GenInfo Identifier: | 74761224 |
NCBI Gene ID: | 84292 |
NCBI Accession: | Q9BRX9.1 |
UniProt Related Accession: | Q9BRX9 |
Molecular Weight: | 34kDa |
NCBI Full Name: | WD repeat domain-containing protein 83 |
NCBI Synonym Full Names: | WD repeat domain 83 |
NCBI Official Symbol: | WDR83 |
NCBI Official Synonym Symbols: | MORG1 |
NCBI Protein Information: | WD repeat domain-containing protein 83 |
UniProt Protein Name: | WD repeat domain-containing protein 83 |
UniProt Synonym Protein Names: | Mitogen-activated protein kinase organizer 1; MAPK organizer 1 |
Protein Family: | WD repeat domain-containing protein |
UniProt Gene Name: | WDR83 |
UniProt Entry Name: | WDR83_HUMAN |