WDR77 Antibody (PACO43402)
- SKU:
- PACO43402
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | WDR77 Antibody (PACO43402) |
抗体コード: | PACO43402 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Methylosome protein 50 protein (1-260AA) |
憲法: | Liquid |
ストレージバッファ: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
精製方法: | Antigen Affinity Purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Western blot. All lanes: Methylosome protein 50 antibody at 6µg/ml. Lane 1: Hela whole cell lysate. Lane 2: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 37, 30 kDa. Observed band size: 37 kDa. |
![]() | Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using PACO43402 at dilution of 1:100. |
![]() | Immunohistochemistry of paraffin-embedded human pancreatic tissue using PACO43402 at dilution of 1:100. |
バックグラウンド: | Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. |
シノニム: | Methylosome protein 50 (MEP-50) (Androgen receptor cofactor p44) (WD repeat-containing protein 77) (p44/Mep50), WDR77, MEP50 WD45 |
UniProt Protein Function: | WDR77: a WD40 repeat protein. Component of the methylosome, a 20S complex containing at least ICLN, SKB1 and WDR77. Interacts with SKB1 and with SM proteins. The methylosome may regulate an early step in the assembly of U snRNPs, possibly the transfer of Sm proteins to the SMN-complex. WD40 repeats are found in a number of eukaryotic proteins that coordinate multi-protein complex assemblies. WD40 proteins are implicated in many functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. |
UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; Adaptor/scaffold; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 1p13.2 Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; cytosol; nucleus Molecular Function:protein binding; ligand-dependent nuclear receptor transcription coactivator activity Biological Process: regulation of transcription from RNA polymerase II promoter; spliceosomal snRNP biogenesis; establishment and/or maintenance of chromatin architecture; positive regulation of cell proliferation; gene expression |
NCBI Summary: | WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).[supplied by OMIM, Mar 2008] |
UniProt Code: | Q9BQA1 |
NCBI GenInfo Identifier: | 32171507 |
NCBI Gene ID: | 79084 |
NCBI Accession: | Q9BQA1.1 |
UniProt Secondary Accession: | Q9BQA1,Q3LID2, Q53FU2, Q6JZZ5, Q96GK4, Q9BWY3, B3KMW6 B4DP38, |
UniProt Related Accession: | Q9BQA1 |
Molecular Weight: | 342 |
NCBI Full Name: | Methylosome protein 50 |
NCBI Synonym Full Names: | WD repeat domain 77 |
NCBI Official Symbol: | WDR77 |
NCBI Official Synonym Symbols: | p44; MEP50; MEP-50; HKMT1069; Nbla10071; p44/Mep50 |
NCBI Protein Information: | methylosome protein 50; androgen receptor cofactor p44; WD repeat-containing protein 77 |
UniProt Protein Name: | Methylosome protein 50 |
UniProt Synonym Protein Names: | Androgen receptor cofactor p44; WD repeat-containing protein 77; p44/Mep50 |
Protein Family: | Methylosome protein |
UniProt Gene Name: | WDR77 |
UniProt Entry Name: | MEP50_HUMAN |