VASN Antibody (PACO63007)
- SKU:
- PACO63007
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | VASN Antibody (PACO63007) |
抗体コード: | PACO63007 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:500-1:1000, IF:1:200-1:500 |
反応性: | Human |
免疫原: | Peptide sequence from Human Vasorin protein (553-572AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: HepG2 whole cell lysate, SH-SY5Y whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate, HL60 whole cell lysate. All lanes: VASN antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 72 kDa. Observed band size: 72 kDa. | |
Immunofluorescence staining of MCF-7 cells with PACO63007 at 1:213, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO63007 diluted at 1:640 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | May act as an inhibitor of TGF-β signaling. |
シノニム: | Vasorin (Protein slit-like 2), VASN, SLITL2 |
UniProt Protein Function: | VASN: May act as an inhibitor of TGF-beta signaling. |
UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: cell surface; extracellular space; lysosomal membrane; plasma membrane Molecular Function:protein binding; transforming growth factor beta binding Biological Process: negative regulation of transforming growth factor beta receptor signaling pathway |
UniProt Code: | Q6EMK4 |
NCBI GenInfo Identifier: | 74748436 |
NCBI Gene ID: | 114990 |
NCBI Accession: | Q6EMK4.1 |
UniProt Secondary Accession: | Q6EMK4,Q6UXL4, Q6UXL5, Q96CX1, |
UniProt Related Accession: | Q6EMK4 |
Molecular Weight: | 71,713 Da |
NCBI Full Name: | Vasorin |
NCBI Synonym Full Names: | vasorin |
NCBI Official Symbol: | VASN |
NCBI Official Synonym Symbols: | SLITL2 |
NCBI Protein Information: | vasorin |
UniProt Protein Name: | Vasorin |
UniProt Synonym Protein Names: | Protein slit-like 2 |
Protein Family: | Vasorin |
UniProt Gene Name: | VASN |
UniProt Entry Name: | VASN_HUMAN |
Antibodies | ELISA Kits |
Anti-VASN Antibody (CAB16215) | Human Vasorin (VASN) ELISA Kit |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |