USP48 Antibody (PACO55998)
- SKU:
- PACO55998
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | USP48 Antibody (PACO55998) |
抗体コード: | PACO55998 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Ubiquitin carboxyl-terminal hydrolase 48 protein (358-508AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO55998 diluted at 1:200 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Western Blot. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, SH-SY5Y whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate. All lanes: USP48 antibody at 3.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 120, 113, 82, 46, 60, 18, 57, 121 kDa. Observed band size: 120 kDa. |
![]() | IHC image of PACO55998 diluted at 1:200 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. May be involved in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at postsynaptic sites. |
シノニム: | Ubiquitin carboxyl-terminal hydrolase 48 (EC 3.4.19.12) (Deubiquitinating enzyme 48) (Ubiquitin thioesterase 48) (Ubiquitin-specific peptidase 48) (Ubiquitin-specific protease 48) (Ubiquitin-specific-processing protease 48), USP48, USP31 |
UniProt Protein Function: | USP48: Recognizes and hydrolyzes the peptide bond at the C- terminal Gly of ubiquitin. Involved in the processing of poly- ubiquitin precursors as well as that of ubiquitinated proteins. May be involved in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at postsynaptic sites. Belongs to the peptidase C19 family. 7 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Ubiquitin conjugating system; Ubiquitin-specific protease; EC 3.4.19.12; Protease Chromosomal Location of Human Ortholog: 1p36.12 Cellular Component: nucleoplasm; mitochondrion; cytoplasm Molecular Function:cysteine-type endopeptidase activity; ubiquitin-specific protease activity Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination |
NCBI Summary: | This gene encodes a protein containing domains that associate it with the peptidase family C19, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. Family members function as deubiquitinating enzymes, recognizing and hydrolyzing the peptide bond at the C-terminal glycine of ubiquitin. Enzymes in peptidase family C19 are involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q86UV5 |
NCBI GenInfo Identifier: | 74750436 |
NCBI Gene ID: | 84196 |
NCBI Accession: | Q86UV5.1 |
UniProt Secondary Accession: | Q86UV5,Q2M3I4, Q5SZI4, Q5T3T5, Q6NX53, Q8N3F6, Q96F64 Q96IQ3, Q9H5N3, Q9H5T7, Q9NUJ6, B7ZKS7, |
UniProt Related Accession: | Q86UV5 |
Molecular Weight: | 1035 |
NCBI Full Name: | Ubiquitin carboxyl-terminal hydrolase 48 |
NCBI Synonym Full Names: | ubiquitin specific peptidase 48 |
NCBI Official Symbol: | USP48 |
NCBI Official Synonym Symbols: | USP31; RAP1GA1 |
NCBI Protein Information: | ubiquitin carboxyl-terminal hydrolase 48; ubiquitin thioesterase 48; deubiquitinating enzyme 48; ubiquitin thiolesterase 48; ubiquitin specific protease 31; ubiquitin specific protease 48; ubiquitin-specific-processing protease 48 |
UniProt Protein Name: | Ubiquitin carboxyl-terminal hydrolase 48 |
UniProt Synonym Protein Names: | Deubiquitinating enzyme 48; Ubiquitin thioesterase 48; Ubiquitin-specific-processing protease 48 |
Protein Family: | Ubiquitin carboxyl-terminal hydrolase |
UniProt Gene Name: | USP48 |
UniProt Entry Name: | UBP48_HUMAN |