UBE2G1 Antibody (PACO58016)
- SKU:
- PACO58016
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | UBE2G1 Antibody (PACO58016) |
抗体コード: | PACO58016 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Ubiquitin-conjugating enzyme E2 G1 protein (2-170AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO58016 diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Immunofluorescence staining of HepG2 cells with PACO58016 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO58016 diluted at 1:500 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4. |
シノニム: | Ubiquitin-conjugating enzyme E2 G1 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme G1) (E217K) (UBC7) (Ubiquitin carrier protein G1) (Ubiquitin-protein ligase G1) [Cleaved into: Ubiquitin-conjugating enzyme E2 G1, N-terminally processed], UBE2G1, UBE2G |
UniProt Protein Function: | UBE2G1: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4. Belongs to the ubiquitin-conjugating enzyme family. |
UniProt Protein Details: | Protein type:EC 6.3.2.19; Ligase; Ubiquitin ligase; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 17p13.2 Cellular Component: cytoplasm Molecular Function:small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity Biological Process: ubiquitin-dependent protein catabolic process; cellular protein catabolic process |
NCBI Summary: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family and catalyzes the covalent attachment of ubiquitin to other proteins. The protein may be involved in degradation of muscle-specific proteins. [provided by RefSeq, Jul 2008] |
UniProt Code: | P62253 |
NCBI GenInfo Identifier: | 51338681 |
NCBI Gene ID: | 7326 |
NCBI Accession: | P62253.3 |
UniProt Secondary Accession: | P62253,Q99462, B2R7P2, D3DTK0, |
UniProt Related Accession: | P62253 |
Molecular Weight: | 170 |
NCBI Full Name: | Ubiquitin-conjugating enzyme E2 G1 |
NCBI Synonym Full Names: | ubiquitin-conjugating enzyme E2G 1 |
NCBI Official Symbol: | UBE2G1 |
NCBI Official Synonym Symbols: | UBC7; E217K; UBE2G |
NCBI Protein Information: | ubiquitin-conjugating enzyme E2 G1; ubiquitin-protein ligase G1; ubiquitin carrier protein G1; ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast); ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, C. elegans); ubiquitin-conjugating enzyme E2G 1 (homologous to C. elegans UBC7) |
UniProt Protein Name: | Ubiquitin-conjugating enzyme E2 G1 |
UniProt Synonym Protein Names: | E217K; UBC7; Ubiquitin carrier protein G1; Ubiquitin-protein ligase G1Ubiquitin-conjugating enzyme E2 G1, N-terminally processed |
Protein Family: | Ubiquitin-conjugating enzyme |
UniProt Gene Name: | UBE2G1 |
UniProt Entry Name: | UB2G1_HUMAN |