TOM1L1 Antibody (PACO56462)
- SKU:
- PACO56462
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | TOM1L1 Antibody (PACO56462) |
抗体コード: | PACO56462 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human TOM1-like protein 1 protein (296-476AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO56462 diluted at 1:240 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Immunofluorescence staining of Hela cells with PACO56462 at 1:105, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | Probable adapter protein involved in signaling pathways. Interacts with the SH2 and SH3 domains of various signaling proteins when it is phosphorylated. May promote FYN activation, possibly by disrupting intramolecular SH3-dependent interactions (By similarity). |
シノニム: | TOM1-like protein 1 (Src-activating and signaling molecule protein) (Target of Myb-like protein 1), TOM1L1, SRCASM |
UniProt Protein Function: | Srcasm: an adapter protein that is a src-family kinase substrate. When phosphorylated, it binds src-family kinases and targets them for lysosomal-dependent degradation. EGF triggers a transient association of phosphorylated Srcasm with EGFR via Grb2 and Shc initiating the endocytosis of the ligand-receptor complex. Interacts with the SH2 and SH3 domains of various signaling proteins when it is phosphorylated. Involved in multivesicular body (MVB) formation, viral egress from the cell, and cytokinesis. Belongs to the TOM1 family. Interacts with FYN, GRB2 and PIK3R1 when phosphorylated. The PTAP sequence in the GAT domain and the PSAP sequence in the C-terminal region are responsible for its interaction with the UEV domain of Tsg101, a component of the ESCRT-1 complex. Localizes to the multivesicular body (MVB) that is involved in targeting endosomal proteins for lysosomal degradation. |
UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 17q23.2 Cellular Component: cytoplasm; cytosol; endosome; lysosome Molecular Function:clathrin binding; protein binding; protein kinase binding; ubiquitin binding Biological Process: activation of protein kinase activity; negative regulation of mitosis; positive regulation of protein amino acid autophosphorylation; signal transduction |
UniProt Code: | O75674 |
NCBI GenInfo Identifier: | 215273903 |
NCBI Gene ID: | 10040 |
NCBI Accession: | O75674.2 |
UniProt Secondary Accession: | O75674,Q53G06, Q8N749, B7Z9E2, |
UniProt Related Accession: | O75674 |
Molecular Weight: | 44,334 Da |
NCBI Full Name: | TOM1-like protein 1 |
NCBI Synonym Full Names: | target of myb1 like 1 membrane trafficking protein |
NCBI Official Symbol: | TOM1L1 |
NCBI Official Synonym Symbols: | SRCASM |
NCBI Protein Information: | TOM1-like protein 1 |
UniProt Protein Name: | TOM1-like protein 1 |
UniProt Synonym Protein Names: | Src-activating and signaling molecule protein; Target of Myb-like protein 1 |
Protein Family: | TOM1-like protein |
UniProt Gene Name: | TOM1L1 |
UniProt Entry Name: | TM1L1_HUMAN |