TKFC Antibody (PACO57172)
- SKU:
- PACO57172
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | TKFC Antibody (PACO57172) |
抗体コード: | PACO57172 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Triokinase/FMN cyclase protein (396-561AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Immunofluorescence staining of HepG2 cells with PACO57172 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO57172 diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. Represses IFIH1-mediated cellular antiviral response. |
シノニム: | Triokinase/FMN cyclase (Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)) [Includes: ATP-dependent dihydroxyacetone kinase (DHA kinase) (EC 2.7.1.28) (EC 2.7.1.29) (Glycerone kinase) (Triokinase) (Triose kinase); FAD-AMP lyase (cyclizing) (EC 4.6.1.15) (FAD-AMP lyase (cyclic FMN forming)) (FMN cyclase)], TKFC, DAK |
UniProt Protein Function: | Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. Represses IFIH1-mediated cellular antiviral response (PubMed:17600090). |
NCBI Summary: | This gene is a member of the family of dihydroxyacetone kinases, which have a protein structure distinct from other kinases. The product of this gene phosphorylates dihydroxyacetone, and also catalyzes the formation of riboflavin 4',5'-phosphate (aka cyclin FMN) from FAD. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2017] |
UniProt Code: | Q3LXA3 |
NCBI GenInfo Identifier: | 20149621 |
NCBI Gene ID: | 26007 |
NCBI Accession: | NP_056348.2 |
UniProt Secondary Accession: | Q3LXA3,Q2L9C1, Q53EQ9, Q9BVA7, Q9H895, |
UniProt Related Accession: | Q3LXA3 |
Molecular Weight: | 54,793 Da |
NCBI Full Name: | triokinase/FMN cyclase isoform b |
NCBI Synonym Full Names: | triokinase and FMN cyclase |
NCBI Official Symbol: | TKFC |
NCBI Official Synonym Symbols: | DAK; NET45 |
NCBI Protein Information: | triokinase/FMN cyclase |
UniProt Protein Name: | Triokinase/FMN cyclase |
UniProt Synonym Protein Names: | Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)Including the following 2 domains:ATP-dependent dihydroxyacetone kinase (EC:2.7.1.28, EC:2.7.1.29); DHA kinaseAlternative name(s):Glycerone kinase; Triokinase; Triose kinase |
Protein Family: | Dihydroxyacetone kinase |
UniProt Gene Name: | TKFC |
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Secondary Antibody |
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