TDG Antibody (PACO43004)
- SKU:
- PACO43004
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | TDG Antibody (PACO43004) |
抗体コード: | PACO43004 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, WB |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:2000 |
反応性: | Human, Mouse |
免疫原: | Recombinant Human G/T mismatch-specific thymine DNA glycosylase protein (141-410AA) |
憲法: | Liquid |
ストレージバッファ: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
精製方法: | Antigen Affinity purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Western blot. All lanes: G/T mismatch-specific thymine DNA glycosylase antibody at 8µg/ml + Mouse brain tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 46 kDa. Observed band size: 46 kDa. |
バックグラウンド: | DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. |
シノニム: | G/T mismatch-specific thymine DNA glycosylase (EC 3.2.2.29) (Thymine-DNA glycosylase) (hTDG), TDG |
UniProt Protein Function: | TDG: In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar- phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. Belongs to the TDG/mug DNA glycosylase family. |
UniProt Protein Details: | Protein type:DNA repair, damage; EC 3.2.2.29; Nuclear receptor co-regulator; Hydrolase Chromosomal Location of Human Ortholog: 12q24.1 Cellular Component: nucleoplasm; PML body; plasma membrane; nucleus Molecular Function:protein domain specific binding; protein binding; protein homodimerization activity; structure-specific DNA binding; SUMO binding; protein kinase C binding; DNA N-glycosylase activity; double-stranded DNA binding; damaged DNA binding; transcription factor binding; pyrimidine-specific mismatch base pair DNA N-glycosylase activity; mismatched DNA binding Biological Process: transcription from RNA polymerase II promoter; embryonic development; mismatch repair; base-excision repair, AP site formation; depyrimidination; base-excision repair; gene expression; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; DNA repair; negative regulation of protein binding; regulation of gene expression, epigenetic |
NCBI Summary: | The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q13569 |
NCBI GenInfo Identifier: | 46397791 |
NCBI Gene ID: | 6996 |
NCBI Accession: | Q13569.2 |
UniProt Secondary Accession: | Q13569,Q8IUZ6, Q8IZM3, |
UniProt Related Accession: | Q13569 |
Molecular Weight: | 410 |
NCBI Full Name: | G/T mismatch-specific thymine DNA glycosylase |
NCBI Synonym Full Names: | thymine-DNA glycosylase |
NCBI Official Symbol: | TDG |
NCBI Official Synonym Symbols: | hTDG |
NCBI Protein Information: | G/T mismatch-specific thymine DNA glycosylase |
UniProt Protein Name: | G/T mismatch-specific thymine DNA glycosylase |
UniProt Synonym Protein Names: | Thymine-DNA glycosylase; hTDG |
Protein Family: | G/T mismatch-specific thymine DNA glycosylase |
UniProt Gene Name: | TDG |
UniProt Entry Name: | TDG_HUMAN |
Antibodies |
Anti-TDG Antibody (CAB1262) |
Anti-TDG Antibody (CAB13365) |
Anti-TDG Antibody (CAB5756) |
TDG Antibody (PACO05186) |
TDG Antibody (PACO12686) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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