TCIRG1 Antibody (PACO59992)
- SKU:
- PACO59992
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | TCIRG1 Antibody (PACO59992) |
抗体コード: | PACO59992 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human V-type proton ATPase 116 kDa subunit a isoform 3 protein (76-182AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO59992 diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Immunofluorescence staining of HepG2 cells with PACO59992 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO59992 diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Part of the proton channel of V-ATPases (By similarity). Seems to be directly involved in T-cell activation. |
シノニム: | V-type proton ATPase 116 kDa subunit a isoform 3 (V-ATPase 116 kDa isoform a3) (Osteoclastic proton pump 116 kDa subunit) (OC-116 kDa) (OC116) (T-cell immune regulator 1) (T-cell immune response cDNA7 protein) (TIRC7) (Vacuolar proton translocating ATPase 116 kDa subunit a isoform 3), TCIRG1, ATP6N1C ATP6V0A3 |
UniProt Protein Function: | Part of the proton channel of V-ATPases. Seems to be directly involved in T-cell activation. |
NCBI Summary: | This gene encodes a subunit of a large protein complex known as a vacuolar H+-ATPase (V-ATPase). The protein complex acts as a pump to move protons across the membrane. This movement of protons helps regulate the pH of cells and their surrounding environment. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. Alternative splicing results in multiple transcript variants. Mutations in this gene are associated with infantile malignant osteopetrosis. [provided by RefSeq, May 2017] |
UniProt Code: | Q13488 |
NCBI GenInfo Identifier: | 19924145 |
NCBI Gene ID: | 10312 |
NCBI Accession: | NP_006010.2 |
UniProt Secondary Accession: | Q13488,O75877, Q8WVC5, |
UniProt Related Accession: | Q13488 |
Molecular Weight: | Calculated: 68kDa; 92kDaObserved: 93kDa |
NCBI Full Name: | V-type proton ATPase 116 kDa subunit a isoform 3 isoform a |
NCBI Synonym Full Names: | T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 |
NCBI Official Symbol: | TCIRG1 |
NCBI Official Synonym Symbols: | a3; Stv1; Vph1; Atp6i; OC116; OPTB1; TIRC7; ATP6N1C; ATP6V0A3; OC-116kDa |
NCBI Protein Information: | V-type proton ATPase 116 kDa subunit a isoform 3; V-type proton ATPase 116 kDa subunit a |
UniProt Protein Name: | V-type proton ATPase 116 kDa subunit a isoform 3 |
UniProt Synonym Protein Names: | Osteoclastic proton pump 116 kDa subunit; OC-116 kDa; OC116; T-cell immune regulator 1; T-cell immune response cDNA7 protein; TIRC7; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 3 |
Protein Family: | V-type proton ATPase |
UniProt Gene Name: | TCIRG1 |