SUN2 Antibody (PACO59469)
- SKU:
- PACO59469
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | SUN2 Antibody (PACO59469) |
抗体コード: | PACO59469 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500 |
反応性: | Human, Mouse, Rat |
免疫原: | Recombinant Human SUN domain-containing protein 2 protein (45-155AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot. Positive WB detected in: Jurkat whole cell lysate, Rat lung tissue, Mouse liver tissue. All lanes: SUN2 antibody at 4.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 81, 83, 80 kDa. Observed band size: 81 kDa. |
![]() | IHC image of PACO59469 diluted at 1:200 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | IHC image of PACO59469 diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Required for nuclear migration in retinal photoreceptor progenitors implicating association with cytoplasmic dynein-dynactin and kinesin motor complexes, and probably B-type lamins; SUN1 and SUN2 seem to act redundantly. The SUN1/2:KASH5 LINC complex couples telomeres to microtubules during meiosis; SUN1 and SUN2 seem to act at least partial redundantly. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. May also function on endocytic vesicles as a receptor for RAB5-GDP and participate in the activation of RAB5. |
シノニム: | SUN domain-containing protein 2 (Protein unc-84 homolog B) (Rab5-interacting protein) (Rab5IP) (Sad1/unc-84 protein-like 2), SUN2, FRIGG KIAA0668 RAB5IP UNC84B |
UniProt Protein Function: | SUN2: a nuclear transmembrane protein. Forms part of a membrane-spanning fibrillar complex that interconnects attached meiotic telomeres with cytoplasmic structures. Together with SUN1 connects the nuclear envelope with the centrosome. Interacts with RAB5A. Reportedly expressed in the endosome where it may play a role in endosome fusion and endocytosis. |
UniProt Protein Details: | Protein type:Membrane protein, integral; Nuclear envelope Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: nuclear envelope; nuclear membrane Molecular Function:identical protein binding; lamin binding; microtubule binding; protein binding Biological Process: centrosome localization; mitotic spindle organization and biogenesis; nuclear membrane organization and biogenesis; nuclear migration; nuclear migration along microfilament; positive regulation of cell migration |
NCBI Summary: | SUN1 (MIM 607723) and SUN2 are inner nuclear membrane (INM) proteins that play a major role in nuclear-cytoplasmic connection by formation of a 'bridge' across the nuclear envelope, known as the LINC complex, via interaction with the conserved luminal KASH domain of nesprins (e.g., SYNE1; MIM 608441) located in the outer nuclear membrane (ONM). The LINC complex provides a direct connection between the nuclear lamina and the cytoskeleton, which contributes to nuclear positioning and cellular rigidity (summary by Haque et al., 2010 [PubMed 19933576]).[supplied by OMIM, Nov 2010] |
UniProt Code: | Q9UH99 |
NCBI GenInfo Identifier: | 29337242 |
NCBI Gene ID: | 25777 |
NCBI Accession: | Q9UH99.3 |
UniProt Secondary Accession: | Q9UH99,O75156, Q2NKN8, Q2T9F7, Q504T5, Q6B4H1, Q7Z3E3 B0QY62, |
UniProt Related Accession: | Q9UH99 |
Molecular Weight: | 79,612 Da |
NCBI Full Name: | SUN domain-containing protein 2 |
NCBI Synonym Full Names: | Sad1 and UNC84 domain containing 2 |
NCBI Official Symbol: | SUN2 |
NCBI Official Synonym Symbols: | UNC84B |
NCBI Protein Information: | SUN domain-containing protein 2 |
UniProt Protein Name: | SUN domain-containing protein 2 |
UniProt Synonym Protein Names: | Protein unc-84 homolog B; Rab5-interacting protein; Rab5IP; Sad1/unc-84 protein-like 2 |
Protein Family: | SUN domain-containing protein |
UniProt Gene Name: | SUN2 |
UniProt Entry Name: | SUN2_HUMAN |
Antibodies |
Anti-SUN2 Antibody (CAB16325) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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