SSRP1 Antibody (PACO46234)
- SKU:
- PACO46234
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | SSRP1 Antibody (PACO46234) |
抗体コード: | PACO46234 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:2000-1:5000, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human FACT complex subunit SSRP1 protein (1-242AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Immunofluorescent analysis of HepG2 cells using PACO46234 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | Western Blot. Positive WB detected in: Hela whole cell lysate, HL60 whole cell lysate, K562 whole cell lysate, A549 whole cell lysate. All lanes: SSRP1 antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 82 kDa. Observed band size: 82 kDa. |
バックグラウンド: | Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63. |
シノニム: | FACT complex subunit SSRP1 (Chromatin-specific transcription elongation factor 80 kDa subunit) (Facilitates chromatin transcription complex 80 kDa subunit) (FACT 80 kDa subunit) (FACTp80) (Facilitates chromatin transcription complex subunit SSRP1) (Recombination signal sequence recognition protein 1) (Structure-specific recognition protein 1) (hSSRP1) (T160), SSRP1, FACT80 |
UniProt Protein Function: | SSRP1: a subunit of the chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63. |
UniProt Protein Details: | Protein type:RNA-binding; Nucleolus; Transcription initiation complex; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 11q12 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:protein binding Biological Process: RNA elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter |
NCBI Summary: | The protein encoded by this gene is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63. An alternatively spliced transcript variant of this gene has been described, but its full-length nature is not known. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q08945 |
NCBI GenInfo Identifier: | 730840 |
NCBI Gene ID: | 6749 |
NCBI Accession: | Q08945.1 |
UniProt Secondary Accession: | Q08945,Q5BJG8, |
UniProt Related Accession: | Q08945 |
Molecular Weight: | 81,075 Da |
NCBI Full Name: | FACT complex subunit SSRP1 |
NCBI Synonym Full Names: | structure specific recognition protein 1 |
NCBI Official Symbol: | SSRP1 |
NCBI Official Synonym Symbols: | FACT; T160; FACT80 |
NCBI Protein Information: | FACT complex subunit SSRP1 |
UniProt Protein Name: | FACT complex subunit SSRP1 |
UniProt Synonym Protein Names: | Chromatin-specific transcription elongation factor 80 kDa subunit; Facilitates chromatin transcription complex 80 kDa subunit; FACT 80 kDa subunit; FACTp80; Facilitates chromatin transcription complex subunit SSRP1; Recombination signal sequence recognition protein 1; Structure-specific recognition protein 1; hSSRP1; T160 |
UniProt Gene Name: | SSRP1 |
UniProt Entry Name: | SSRP1_HUMAN |