SPO11 Antibody (PACO59577)
- SKU:
- PACO59577
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | SPO11 Antibody (PACO59577) |
抗体コード: | PACO59577 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000 |
反応性: | Human |
免疫原: | Recombinant Human Meiotic recombination protein SPO11 protein (28-109AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot. Positive WB detected in: Jurkat whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate. All lanes: SPO11 antibody at 5.8µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 45, 41 kDa. Observed band size: 45 kDa. |
バックグラウンド: | Component of a topoisomerase 6 complex specifically required for meiotic recombination. Together with TOP6BL, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles. Essential for the phosphorylation of SMC3, HORMAD1 and HORMAD2. |
シノニム: | Meiotic recombination protein SPO11 (EC 5.99.1.3) (Cancer/testis antigen 35) (CT35), SPO11 |
UniProt Protein Function: | SPO11: Required for meiotic recombination. Mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. Belongs to the TOP6A family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Cell cycle regulation; Cancer Testis Antigen (CTA); Cell development/differentiation Chromosomal Location of Human Ortholog: 20q13.31 Molecular Function:protein binding Biological Process: female gamete generation; spermatogenesis |
NCBI Summary: | Meiotic recombination and chromosome segregation require the formation of double-strand breaks (DSBs) in paired chromosome homologs. During meiosis in yeast, a meiotic recombination protein is covalently-linked to the 5' end of DSBs and is essential for the formation of DSBs. The protein encoded by this gene is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been described. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q9Y5K1 |
NCBI GenInfo Identifier: | 7674367 |
NCBI Gene ID: | 23626 |
NCBI Accession: | Q9Y5K1.1 |
UniProt Secondary Accession: | Q9Y5K1,Q5TCI1, Q8N4V0, Q9NQM7, Q9NQM8, |
UniProt Related Accession: | Q9Y5K1 |
Molecular Weight: | 40,364 Da |
NCBI Full Name: | Meiotic recombination protein SPO11 |
NCBI Synonym Full Names: | SPO11, initiator of meiotic double stranded breaks |
NCBI Official Symbol: | SPO11 |
NCBI Official Synonym Symbols: | CT35; TOPVIA; SPATA43 |
NCBI Protein Information: | meiotic recombination protein SPO11 |
UniProt Protein Name: | Meiotic recombination protein SPO11 |
UniProt Synonym Protein Names: | Cancer/testis antigen 35; CT35 |
Protein Family: | Meiosis-specific protein |
UniProt Gene Name: | SPO11 |
UniProt Entry Name: | SPO11_HUMAN |