SMPD2 Antibody (PACO21091)
- SKU:
- PACO21091
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | SMPD2 Antibody (PACO21091) |
抗体コード: | PACO21091 |
サイズ: | 100ul(100ug) |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:50-1:200 |
反応性: | Human, Mouse, Rat |
免疫原: | Recombinant protein of human SMPD2 |
憲法: | Liquid |
ストレージバッファ: | Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
精製方法: | Affinity purification |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Western blot analysis of brian cell lysate using SMPD2 antibody. |
バックグラウンド: | Sphingomyelinases (SMases) catalyze the hydrolysis of sphingomyelin to produce ceramide and phosphocholine. Ceramide is an important bioactive lipid triggering signal transduction involved in cell proliferation, apoptosis and differentiation. A number of SMases have been described and categorized based on their optimum pH activity, cation dependence, tissue distribution, and subcellular localization. These include a lysosomal acid, SMase, a Zn++-dependent secreted acid, SMase, a membrane-bound Mg++-dependent neutral SMase, a Mg++-independent neutral SMase, and an alkaline SMase.nSMase1 (also termed SMPD2) is a Mg++-dependent neutral SMase that is widely expressed and predominantly localized to the endoplasmic reticulum. This protein has also been shown to have lyso-platelet activating factor (PAF) phospholipase C activity. A second neutral SMase, nSMase2 (also termed SMPD3) is predominantly expressed in the brain. The activity of neutral SMases is regulated by oxidative stress, chemotherapeutic drugs, inflammatory cytokines, and apoptotic stimuli. Analysis of single and double knockouts of the SMPD2 and SMPD3 has revealed that loss of both genes leads to complete loss of neutral SMase activity with developmental defects observed with loss of nSMase2. |
シノニム: | SMPD2;NSMASE;NSMASE1 |
UniProt Protein Function: | SMPD2: Converts sphingomyelin to ceramide. Hydrolyze 1-acyl-2- lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso- sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF. Belongs to the neutral sphingomyelinase family. |
UniProt Protein Details: | Protein type:Membrane protein, integral; Phosphodiesterase; Lipid Metabolism - sphingolipid; Membrane protein, multi-pass; EC 3.1.4.12 Chromosomal Location of Human Ortholog: 6q21 Cellular Component: integral to plasma membrane; plasma membrane; caveola Molecular Function:sphingomyelin phosphodiesterase activity; metal ion binding Biological Process: sphingolipid metabolic process; sphingomyelin metabolic process; nerve growth factor receptor signaling pathway; response to mechanical stimulus; ceramide biosynthetic process; glycosphingolipid metabolic process |
NCBI Summary: | This gene encodes a protein which was initially identified as a sphingomyelinase based on sequence similarity between bacterial sphingomyelinases and a yeast protein. Subsequent studies showed that its biological function is less likely to be as a sphingomyelinase and instead as a lysophospholipase. [provided by RefSeq, Oct 2009] |
UniProt Code: | O60906 |
NCBI GenInfo Identifier: | 160332206 |
NCBI Gene ID: | 6610 |
NCBI Accession: | O60906.2 |
UniProt Related Accession: | O60906 |
Molecular Weight: | 48kDa |
NCBI Full Name: | Sphingomyelin phosphodiesterase 2 |
NCBI Synonym Full Names: | sphingomyelin phosphodiesterase 2 |
NCBI Official Symbol: | SMPD2 |
NCBI Official Synonym Symbols: | ISC1; NSMASE; NSMASE1 |
NCBI Protein Information: | sphingomyelin phosphodiesterase 2 |
UniProt Protein Name: | Sphingomyelin phosphodiesterase 2 |
UniProt Synonym Protein Names: | Lyso-platelet-activating factor-phospholipase C; Lyso-PAF-PLC; Neutral sphingomyelinase; N-SMase; nSMase |
UniProt Gene Name: | SMPD2 |
UniProt Entry Name: | NSMA_HUMAN |
Antibodies |
Anti-SMPD2 Antibody (CAB1166) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |