SHARPIN Antibody, FITC conjugated (PACO57466)
- SKU:
- PACO57466
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Antibody type:
- Polyclonal
- Conjugation:
- FITC
Description
抗体名: | SHARPIN Antibody, FITC conjugated (PACO57466) |
抗体コード: | PACO57466 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA |
推奨される希釈: | |
反応性: | Human |
免疫原: | Recombinant Human Sharpin protein (82-171AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | FITC |
バックグラウンド: | Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. |
シノニム: | Sharpin (Shank-associated RH domain-interacting protein) (Shank-interacting protein-like 1) (hSIPL1), SHARPIN, SIPL1 |
UniProt Protein Function: | Function: Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Ref.10 Ref.11 Ref.12 |
UniProt Protein Details: | Subunit structure: Monomer and homodimer. Interacts with SHANK1, EYA1 and EYA2 By similarity. Component of the LUBAC complex (linear ubiquitin chain assembly complex) which consists of SHARPIN, RBCK1 and RNF31. LUBAC has a MW of approximative 600 kDa suggesting a heteromultimeric assembly of its subunits. Associates with the TNF-R1 signaling complex (TNF-RSC) in a stimulation-dependent manner. Ref.10 Ref.11 Ref.12 Ref.13 Subcellular location: Cytoplasm › cytosol Ref.2. Tissue specificity: Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Up-regulated in various tumor tissues such as kidney, liver, ovary and pancreas tumors. Ref.2 Domain: The Ubiquitin-like domain is required for the interaction with RNF31. Ref.10 Ref.11 Ref.12The RanBP2-type zinc fingers mediate the specific interaction with ubiquitin. Binds preferentially linear polyubiquitin chains and 'Lys-63'-linked polyubiquitin chains over 'Lys-48'-linked polyubiquitin chains. Also binds monoubiquitin. Ref.10 Ref.11 Ref.12 Sequence similarities: Contains 1 RanBP2-type zinc finger.Contains 1 ubiquitin-like domain. Sequence caution: The sequence BAC11666.1 differs from that shown. Reason: Frameshift at position 334. The sequence BAC53797.1 differs from that shown. Reason: Frameshift at position 218. The sequence BAC53798.1 differs from that shown. Reason: Aberrant splicing. |
UniProt Code: | Q9H0F6 |
NCBI GenInfo Identifier: | 118918415 |
NCBI Gene ID: | 81858 |
NCBI Accession: | NP_112236.3 |
UniProt Secondary Accession: | Q9H0F6,Q8IXF5, Q8IXF6, Q8N2E7, Q8TB25, Q9BUE4, A6NEG3 C0L3L2, D3DWL3, |
UniProt Related Accession: | Q9H0F6 |
Molecular Weight: | 39,949 Da |
NCBI Full Name: | sharpin |
NCBI Synonym Full Names: | SHANK-associated RH domain interactor |
NCBI Official Symbol: | SHARPIN |
NCBI Official Synonym Symbols: | SIPL1 |
NCBI Protein Information: | sharpin; hSIPL1; shank-interacting protein-like 1; shank-associated RH domain-interacting protein |
UniProt Protein Name: | Sharpin |
UniProt Synonym Protein Names: | Shank-associated RH domain-interacting protein; Shank-interacting protein-like 1 |
Protein Family: | Sharpin |
UniProt Gene Name: | SHARPIN |
UniProt Entry Name: | SHRPN_HUMAN |