RPA2 Antibody, Biotin conjugated (PACO28513)
- SKU:
- PACO28513
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Antibody type:
- Polyclonal
- Conjugation:
- Biotin
Description
抗体名: | RPA2 Antibody, Biotin conjugated (PACO28513) |
抗体コード: | PACO28513 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA |
推奨される希釈: | |
反応性: | Human |
免疫原: | Recombinant Human Replication protein A 32 kDa subunit protein (1-267AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Biotin |
バックグラウンド: | Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase α but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. |
シノニム: | Replication protein A 32 kDa subunit (RP-A p32) (Replication factor A protein 2) (RF-A protein 2) (Replication protein A 34 kDa subunit) (RP-A p34), RPA2, REPA2 RPA32 RPA34 |
UniProt Protein Function: | RFA2: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage. Heterotrimer of 70, 32 and 14 kDa chains (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN. Directly interacts with PPP4R2, but not with SMEK2; this interaction is DNA damage-dependent and leads RPA2 dephosphorylation by PPP4C recruitment. Interacts with RAD51, preferentially when hyperphosphorylated. Directly interacts with RFWD3. 3 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:DNA replication Chromosomal Location of Human Ortholog: 1p35 Cellular Component: chromatin; chromosome, telomeric region; DNA replication factor A complex; nuclear chromosome, telomeric region; nucleoplasm; nucleus; PML body Molecular Function:damaged DNA binding; enzyme binding; protein binding; protein N-terminus binding; protein phosphatase binding; single-stranded DNA binding; ubiquitin protein ligase binding Biological Process: base-excision repair; bypass DNA synthesis; DNA damage response, detection of DNA damage; DNA repair; DNA replication; DNA strand elongation during DNA replication; double-strand break repair; double-strand break repair via homologous recombination; error-prone postreplication DNA repair; G1 DNA damage checkpoint; G1/S transition of mitotic cell cycle; mismatch repair; mitotic cell cycle; nucleotide-excision repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; telomere maintenance; telomere maintenance via recombination; telomere maintenance via semi-conservative replication; transcription-coupled nucleotide-excision repair |
UniProt Code: | P15927 |
NCBI GenInfo Identifier: | 132474 |
NCBI Gene ID: | 6118 |
NCBI Accession: | P15927.1 |
UniProt Secondary Accession: | P15927,Q52II0, Q5TEI9, Q5TEJ5, |
UniProt Related Accession: | P15927 |
Molecular Weight: | 38,810 Da |
NCBI Full Name: | Replication protein A 32 kDa subunit |
NCBI Synonym Full Names: | replication protein A2 |
NCBI Official Symbol: | RPA2 |
NCBI Official Synonym Symbols: | REPA2; RPA32; RP-A p32; RP-A p34 |
NCBI Protein Information: | replication protein A 32 kDa subunit |
UniProt Protein Name: | Replication protein A 32 kDa subunit |
UniProt Synonym Protein Names: | Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RP-A p34 |
Protein Family: | Replication protein |
UniProt Gene Name: | RPA2 |
UniProt Entry Name: | RFA2_HUMAN |