RNF128 Antibody (PACO38618)
- SKU:
- PACO38618
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | RNF128 Antibody (PACO38618) |
抗体コード: | PACO38618 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IF:1:50-1:200 |
反応性: | Human, Mouse |
免疫原: | Recombinant Human E3 ubiquitin-protein ligase RNF128 protein (229-400AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Western blot. All lanes: RNF128 antibody at 12µg/ml + Mouse liver tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 47, 45 kDa. Observed band size: 47 kDa. |
![]() | Immunofluorescent analysis of HepG2 cells using PACO38618 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | E3 ubiquitin-protein ligase that catalyzes 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains formation. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription, thereby playing an important role in the induction of the anergic phenotype, a long-term stable state of T-lymphocyte unresponsiveness to antigenic stimulation associated with the blockade of interleukin production. Ubiquitinates ARPC5 with 'Lys-48' linkages and COR1A with 'Lys-63' linkages leading to their degradation, down-regulation of these cytosleletal components results in impaired lamellipodium formation and reduced accumulation of F-actin at the immunological synapse. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals. |
シノニム: | E3 ubiquitin-protein ligase RNF128 (EC 2.3.2.27) (Gene related to anergy in lymphocytes protein) (GRAIL) (RING finger protein 128) (RING-type E3 ubiquitin transferase RNF128), RNF128 |
UniProt Protein Function: | RNF128: E3 ubiquitin-protein ligase that catalyzes polyubiquitin chains. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription and this activity is likely to be mediated by E3 ligase activity. Plays an important role in the induction of the anergic phenotype. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural- inducing signals. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:EC 6.3.2.19; EC 6.3.2.-; Ligase; Membrane protein, integral; Ubiquitin ligase; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: Xq22.3 Molecular Function:protein binding Biological Process: regulation of protein stability |
NCBI Summary: | The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q8TEB7 |
NCBI GenInfo Identifier: | 74751443 |
NCBI Gene ID: | 79589 |
NCBI Accession: | Q8TEB7.1 |
UniProt Secondary Accession: | Q8TEB7,Q6PH80, Q6ZTJ8, Q96RF3, Q9H5E4, A0PJI4, |
UniProt Related Accession: | Q8TEB7 |
Molecular Weight: | 44,617 Da |
NCBI Full Name: | E3 ubiquitin-protein ligase RNF128 |
NCBI Synonym Full Names: | ring finger protein 128, E3 ubiquitin protein ligase |
NCBI Official Symbol: | RNF128 |
NCBI Official Synonym Symbols: | GRAIL |
NCBI Protein Information: | E3 ubiquitin-protein ligase RNF128 |
UniProt Protein Name: | E3 ubiquitin-protein ligase RNF128 |
UniProt Synonym Protein Names: | Gene related to anergy in lymphocytes protein; GRAIL; RING finger protein 128 |
Protein Family: | E3 ubiquitin-protein ligase |
UniProt Gene Name: | RNF128 |
UniProt Entry Name: | RN128_HUMAN |
Antibodies |
Anti-RNF128 Antibody (CAB18276) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |