RAD9A Antibody, FITC conjugated (PACO54180)
- SKU:
- PACO54180
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Antibody type:
- Polyclonal
- Conjugation:
- FITC
Frequently bought together:
Description
抗体名: | RAD9A Antibody, FITC conjugated (PACO54180) |
抗体コード: | PACO54180 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA |
推奨される希釈: | |
反応性: | Human |
免疫原: | Recombinant Human Cell cycle checkpoint control protein RAD9A protein (267-341AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | FITC |
バックグラウンド: | Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase β (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex. |
シノニム: | Cell cycle checkpoint control protein RAD9A (hRAD9) (EC 3.1.11.2) (DNA repair exonuclease rad9 homolog A), RAD9A |
UniProt Protein Function: | Rad9: a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. Possesses a 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. Forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for Rad9. |
UniProt Protein Details: | Protein type:EC 3.1.11.2; DNA replication; Deoxyribonuclease; Apoptosis; Cell cycle regulation Chromosomal Location of Human Ortholog: 11q13.1-q13.2 Cellular Component: nucleoplasm; cytoplasm; nucleus Molecular Function:protein binding; enzyme binding; histone deacetylase binding; exodeoxyribonuclease III activity; 3'-5' exonuclease activity; SH3 domain binding; protein kinase binding Biological Process: intra-S DNA damage checkpoint; DNA damage checkpoint; DNA replication checkpoint; DNA replication; DNA repair; DNA catabolic process, exonucleolytic; response to DNA damage stimulus |
NCBI Summary: | This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
UniProt Code: | Q99638 |
NCBI GenInfo Identifier: | 74717382 |
NCBI Gene ID: | 5883 |
NCBI Accession: | Q99638.1 |
UniProt Related Accession: | Q99638 |
Molecular Weight: | |
NCBI Full Name: | Cell cycle checkpoint control protein RAD9A |
NCBI Synonym Full Names: | RAD9 checkpoint clamp component A |
NCBI Official Symbol: | RAD9A |
NCBI Official Synonym Symbols: | RAD9 |
NCBI Protein Information: | cell cycle checkpoint control protein RAD9A |
UniProt Protein Name: | Cell cycle checkpoint control protein RAD9A |
UniProt Synonym Protein Names: | DNA repair exonuclease rad9 homolog A |
Protein Family: | RAD9, HUS1, RAD1-interacting nuclear orphan protein |
UniProt Gene Name: | RAD9A |
UniProt Entry Name: | RAD9A_HUMAN |