RAB25 Antibody (PACO29316)
- SKU:
- PACO29316
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | RAB25 Antibody (PACO29316) |
抗体コード: | PACO29316 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
反応性: | Human, Mouse |
免疫原: | Recombinant Human Ras-related protein Rab-25 protein (1-210AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot Positive WB detected in: MCF-7 whole cell lysate, Mouse spleen tissue, Mouse lung tissue, Mouse brain tissue All lanes: RAB25 antibody at 4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 24 kDa Observed band size: 24 kDa . |
![]() | Immunofluorescent analysis of Hela cells using PACO29316 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | Immunohistochemistry of paraffin-embedded human testis tissue using PACO29316 at dilution of 1:100. |
バックグラウンド: | Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia (PubMed:17925226). Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions. May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis. |
シノニム: | Ras-related protein Rab-25 (CATX-8), RAB25, CATX8 |
UniProt Protein Function: | RAB25: Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia. Increases the rates of ovarian and breast cancers progression and aggressiveness by modulating cellular processes such as proliferation, survival and migration of epithelial tumor cells. May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis. Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4. Interacts (via the hypervariable C-terminal region) with ITGB1 (via the cytoplasmic region); the interaction is GTP- dependent. Interacts with ITGAV. Associates with the integrin alpha-V/beta-1 heterodimer. Expressed in ovarian epithelium (NOE) and breast tissue. Expressed in ovarian cancer; expression is increased relative to NOE cells. Expression in ovarian cancer is stage dependent, with stage III and stage IV showing higher levels than early stage cancers. Expressed in breast cancer; expression is increased relative to normal breast tissue. Belongs to the small GTPase superfamily. Rab family. |
UniProt Protein Details: | Protein type:G protein, monomeric; G protein, monomeric, Rab Chromosomal Location of Human Ortholog: 1q22 Cellular Component: cytoplasmic vesicle; pseudopodium Molecular Function:myosin V binding; protein binding Biological Process: positive regulation of cell proliferation; pseudopodium organization and biogenesis |
NCBI Summary: | The protein encoded by this gene is a member of the RAS superfamily of small GTPases. The encoded protein is involved in membrane trafficking and cell survival. This gene has been found to be a tumor suppressor and an oncogene, depending on the context. Two variants, one protein-coding and the other not, have been found for this gene. [provided by RefSeq, Nov 2015] |
UniProt Code: | P57735 |
NCBI GenInfo Identifier: | 46577696 |
NCBI Gene ID: | 57111 |
NCBI Accession: | P57735.2 |
UniProt Secondary Accession: | P57735,Q5VYA2, Q8NG24, Q96GB1, Q9BT12, |
UniProt Related Accession: | P57735 |
Molecular Weight: | 23kDa |
NCBI Full Name: | Ras-related protein Rab-25 |
NCBI Synonym Full Names: | RAB25, member RAS oncogene family |
NCBI Official Symbol: | RAB25 |
NCBI Official Synonym Symbols: | CATX-8; RAB11C |
NCBI Protein Information: | ras-related protein Rab-25 |
UniProt Protein Name: | Ras-related protein Rab-25 |
UniProt Synonym Protein Names: | CATX-8 |
Protein Family: | Ras-related protein |
UniProt Gene Name: | RAB25 |
Antibodies |
Anti-RAB25 Antibody (CAB12275) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-HRP-conjugated Beta Actin Antibody (CABC028) |