PSMC6 Antibody (PACO29232)
- SKU:
- PACO29232
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | PSMC6 Antibody (PACO29232) |
抗体コード: | PACO29232 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:2000 |
反応性: | Human, Mouse, Rat |
免疫原: | Recombinant Human 26S proteasome regulatory subunit 10B protein (1-260AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Mouse brain tissue, Mouse liver tissue, Mouse kidney tissue, Rat brain tissue, Rat liver tissue, Rat kidney tissue All lanes: PSMC6 antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa . |
![]() | IHC image of PACO29232 diluted at 1:600 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | IHC image of PACO29232 diluted at 1:1200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. |
シノニム: | 26S proteasome regulatory subunit 10B (26S proteasome AAA-ATPase subunit RPT4) (Proteasome 26S subunit ATPase 6) (Proteasome subunit p42), PSMC6, SUG2 |
UniProt Protein Function: | RPT4: The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. Belongs to the AAA ATPase family. |
UniProt Protein Details: | Protein type:Proteasome complex; Protease Chromosomal Location of Human Ortholog: 14q22.1 Cellular Component: nucleoplasm; proteasome complex; membrane; nucleus; cytosol Molecular Function:protein binding, bridging; protein binding; ATPase activity; ATP binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle |
NCBI Summary: | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. Pseudogenes have been identified on chromosomes 8 and 12. [provided by RefSeq, Jul 2008] |
UniProt Code: | P62333 |
NCBI GenInfo Identifier: | 51702772 |
NCBI Gene ID: | 5706 |
NCBI Accession: | P62333.1 |
UniProt Secondary Accession: | P62333,P49719, Q6IBU3, Q92524, B2R975, |
UniProt Related Accession: | P62333 |
Molecular Weight: | 389 |
NCBI Full Name: | 26S protease regulatory subunit 10B |
NCBI Synonym Full Names: | proteasome (prosome, macropain) 26S subunit, ATPase, 6 |
NCBI Official Symbol: | PSMC6 |
NCBI Official Synonym Symbols: | P44; p42; SUG2; CADP44; HEL-S-73 |
NCBI Protein Information: | 26S protease regulatory subunit 10B; proteasome subunit p42; proteasome 26S subunit ATPase 6; conserved ATPase domain protein 44; epididymis secretory protein Li 73; 26S protease regulatory subunit S10B; 26S proteasome AAA-ATPase subunit RPT4 |
UniProt Protein Name: | 26S protease regulatory subunit 10B |
UniProt Synonym Protein Names: | 26S proteasome AAA-ATPase subunit RPT4; Proteasome 26S subunit ATPase 6; Proteasome subunit p42 |
Protein Family: | 26S protease regulatory |
UniProt Gene Name: | PSMC6 |
UniProt Entry Name: | PRS10_HUMAN |
Antibodies | ELISA Kits |
Anti-PSMC6 Antibody (CAB5377) | Human PSMC6 (Proteasome 26S subunit ATPase 6) ELISA Kit (HUFI06994) |
PSMC6 Antibody (PACO01371) | |
PSMC6 Antibody (PACO11544) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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