PRPF8 Antibody (PACO58200)
- SKU:
- PACO58200
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | PRPF8 Antibody (PACO58200) |
抗体コード: | PACO58200 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Pre-mRNA-processing-splicing factor 8 protein (2037-2170AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Immunofluorescence staining of Hela cells with PACO58200 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site. |
シノニム: | Pre-mRNA-processing-splicing factor 8 (220 kDa U5 snRNP-specific protein) (PRP8 homolog) (Splicing factor Prp8) (p220), PRPF8, PRPC8 |
UniProt Protein Function: | Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site. |
NCBI Summary: | Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q6P2Q9 |
NCBI GenInfo Identifier: | 67460824 |
NCBI Gene ID: | 10594 |
NCBI Accession: | Q6P2Q9.2 |
UniProt Related Accession: | Q6P2Q9 |
Molecular Weight: | |
NCBI Full Name: | Pre-mRNA-processing-splicing factor 8 |
NCBI Synonym Full Names: | pre-mRNA processing factor 8 |
NCBI Official Symbol: | PRPF8 |
NCBI Official Synonym Symbols: | PRP8; RP13; HPRP8; PRPC8; SNRNP220 |
NCBI Protein Information: | pre-mRNA-processing-splicing factor 8 |
UniProt Protein Name: | Pre-mRNA-processing-splicing factor 8 |
UniProt Synonym Protein Names: | 220 kDa U5 snRNP-specific protein; PRP8 homolog; Splicing factor Prp8; p220 |
Protein Family: | Pre-mRNA-splicing factor |
UniProt Gene Name: | PRPF8 |