POLD2 Antibody, FITC conjugated (PACO57926)
- SKU:
- PACO57926
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Antibody type:
- Polyclonal
- Conjugation:
- FITC
Frequently bought together:
Description
抗体名: | POLD2 Antibody, FITC conjugated (PACO57926) |
抗体コード: | PACO57926 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA |
推奨される希釈: | |
反応性: | Human |
免疫原: | Recombinant Human DNA polymerase delta subunit 2 protein (245-430AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | FITC |
バックグラウンド: | As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair. Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion. Also involved in TLS as a component of the POLZ complex. Along with POLD3, dramatically increases the efficiency and processivity of DNA synthesis of the minimal DNA polymerase zeta complex, consisting of only REV3L and REV7. |
シノニム: | DNA polymerase delta subunit 2 (DNA polymerase delta subunit p50), POLD2 |
UniProt Protein Function: | POLD2: The function of the small subunit is not yet clear. Belongs to the DNA polymerase delta/II small subunit family. |
UniProt Protein Details: | Protein type:DNA replication; EC 2.7.7.7; Nucleotide Metabolism - pyrimidine; DNA repair, damage; Nucleotide Metabolism - purine; Transferase Chromosomal Location of Human Ortholog: 7p13 Cellular Component: nucleoplasm; nucleus Molecular Function:DNA-directed DNA polymerase activity; protein binding Biological Process: bypass DNA synthesis; DNA damage response, detection of DNA damage; DNA replication; DNA strand elongation during DNA replication; mismatch repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 5'-to lesion; telomere maintenance; telomere maintenance via recombination; transcription-coupled nucleotide-excision repair |
NCBI Summary: | This gene encodes the 50-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein is required for the stimulation of DNA polymerase delta activity by the processivity cofactor proliferating cell nuclear antigen (PCNA). Expression of this gene may be a marker for ovarian carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Mar 2012] |
UniProt Code: | P49005 |
NCBI GenInfo Identifier: | 1352307 |
NCBI Gene ID: | 5425 |
NCBI Accession: | P49005.1 |
UniProt Secondary Accession: | P49005,A4D2J4, B2R5S4, |
UniProt Related Accession: | P49005 |
Molecular Weight: | 51,289 Da |
NCBI Full Name: | DNA polymerase delta subunit 2 |
NCBI Synonym Full Names: | DAN polymerase delta 2, accessory subunit |
NCBI Official Symbol: | POLD2 |
NCBI Protein Information: | DNA polymerase delta subunit 2 |
UniProt Protein Name: | DNA polymerase delta subunit 2 |
UniProt Synonym Protein Names: | DNA polymerase delta subunit p50 |
Protein Family: | DNA polymerase |
UniProt Gene Name: | POLD2 |
UniProt Entry Name: | DPOD2_HUMAN |