PLXNB1 Antibody (PACO56358)
- SKU:
- PACO56358
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | PLXNB1 Antibody (PACO56358) |
抗体コード: | PACO56358 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Plexin-B1 protein (664-837AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO56358 diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Immunofluorescence staining of Hela cells with PACO56358 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO56358 diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Receptor for SEMA4D. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. |
シノニム: | Plexin-B1 (Semaphorin receptor SEP), PLXNB1, KIAA0407 PLXN5 SEP |
UniProt Protein Function: | PLXNB1: Receptor for SEMA4D. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Belongs to the plexin family. 3 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Membrane protein, integral; Receptor, misc.; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 3p21.31 Cellular Component: integral to plasma membrane; extracellular region; plasma membrane; intracellular Molecular Function:protein binding; transmembrane receptor activity; semaphorin receptor activity; GTPase activating protein binding; receptor activity; semaphorin receptor binding Biological Process: positive regulation of phosphoinositide 3-kinase cascade; regulation of cell shape; axon guidance; cell migration; positive regulation of axonogenesis; negative regulation of osteoblast proliferation; negative regulation of cell adhesion; signal transduction; neurite morphogenesis; regulation of cytoskeleton organization and biogenesis |
UniProt Code: | O43157 |
NCBI GenInfo Identifier: | 194272180 |
NCBI Gene ID: | 5364 |
NCBI Accession: | NP_001123554.1 |
UniProt Secondary Accession: | O43157,Q6NY20, Q9UIV7, Q9UJ92, Q9UJ93, A6H8Y2, |
UniProt Related Accession: | O43157 |
Molecular Weight: | 232,298 Da |
NCBI Full Name: | plexin-B1 |
NCBI Synonym Full Names: | plexin B1 |
NCBI Official Symbol: | PLXNB1 |
NCBI Official Synonym Symbols: | SEP; PLXN5; KIAA0407; MGC149167; PLEXIN-B1 |
NCBI Protein Information: | plexin-B1; plexin 5; OTTHUMP00000164806; OTTHUMP00000210032; OTTHUMP00000210033; semaphorin receptor SEP |
UniProt Protein Name: | Plexin-B1 |
UniProt Synonym Protein Names: | Semaphorin receptor SEP |
Protein Family: | Plexin |
UniProt Gene Name: | PLXNB1 |
UniProt Entry Name: | PLXB1_HUMAN |