PIN4 Antibody (PACO58448)
- SKU:
- PACO58448
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | PIN4 Antibody (PACO58448) |
抗体コード: | PACO58448 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500 |
反応性: | Human, Mouse, Rat |
免疫原: | Recombinant Human Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 protein (1-131AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot. Positive WB detected in: 293T whole cell lysate, RAW264.7 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue, Mouse liver tissue. All lanes: PIN4 antibody at 5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 14, 17 kDa. Observed band size: 14 kDa. |
![]() | Immunofluorescence staining of HepG2 cells with PACO58448 at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO58448 diluted at 1:800 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA. |
シノニム: | Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 (EC 5.2.1.8) (Parvulin-14) (Par14) (hPar14) (Parvulin-17) (Par17) (hPar17) (Peptidyl-prolyl cis-trans isomerase Pin4) (PPIase Pin4) (Peptidyl-prolyl cis/trans isomerase EPVH) (hEPVH) (Rotamase Pin4), PIN4 |
UniProt Protein Function: | PIN4: Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA. Belongs to the PpiC/parvulin rotamase family. PIN4 subfamily. 2 isoforms of the human protein are produced by alternative promoter. |
UniProt Protein Details: | Protein type:Nucleolus; Isomerase; Mitochondrial; EC 5.2.1.8 Chromosomal Location of Human Ortholog: Xq13 Molecular Function:protein binding |
NCBI Summary: | This gene encodes a member of the parvulin subfamily of the peptidyl-prolyl cis/trans isomerase protein family. The encoded protein catalyzes the isomerization of peptidylprolyl bonds, and may play a role in the cell cycle, chromatin remodeling, and/or ribosome biogenesis. The encoded protein may play an additional role in the mitochondria. [provided by RefSeq, Dec 2009] |
UniProt Code: | Q9Y237 |
NCBI GenInfo Identifier: | 20139299 |
NCBI Gene ID: | 5303 |
NCBI Accession: | Q9Y237.1 |
UniProt Secondary Accession: | Q9Y237,Q0D2H3, Q3MHV0, Q52M21, Q5HYW6, Q6IRW4, A8E0G6 B3KXM0, F5H1P5, |
UniProt Related Accession: | Q9Y237 |
Molecular Weight: | 14,179 Da |
NCBI Full Name: | Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 |
NCBI Synonym Full Names: | peptidylprolyl cis/trans isomerase, NIMA-interacting 4 |
NCBI Official Symbol: | PIN4 |
NCBI Official Synonym Symbols: | EPVH; PAR14; PAR17 |
NCBI Protein Information: | peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 |
UniProt Protein Name: | Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 |
UniProt Synonym Protein Names: | Parvulin-14; Par14; hPar14; Parvulin-17; Par17; hPar17; Peptidyl-prolyl cis-trans isomerase Pin4; PPIase Pin4; Peptidyl-prolyl cis/trans isomerase EPVH; hEPVH; Rotamase Pin4 |
Protein Family: | Peptidyl-prolyl cis-trans isomerase |
UniProt Gene Name: | PIN4 |
UniProt Entry Name: | PIN4_HUMAN |
Antibodies |
Anti-PIN4 Antibody (CAB18173) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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